Marine Ecology. 2019;40:e12544. wileyonlinelibrary.com/journal/maec
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https://doi.org/10.1111/maec.12544
© 2019 Blackwell Verlag GmbH
1 | INTRODUCTION
Mangroves are coastal ecosystems found in tropical and subtropical
regions around the world. They are found in the transitional zones
between land, sea, and rivers; regarding their geographical distribu‐
tion, these ecosystems are found in the Americas, Africa, Asia, and
Oceania (Brito et al., 2006). Mangroves can tolerate a wide range
of sediment types, temperature changes, nutrient, salinity, and ox‐
ygen levels. Mangrove plant species vary in their tolerance to these
factors, forming characteristic templates or zones of vegetation
(Lovelock, 1993). This ecosystem provides various natural func‐
tions of great ecological and economic importance. Some of these
functions include being an important nursery for fish, crustaceans,
mollusks, reptiles, mammals, bird nesting, a site of carbon and nutri‐
ent accumulation, a location of marine biomass reconstruction and
offering protection against coastal erosion (Alongi, 2002).
Mangrove forests were first recorded in the Persian Gulf
and Oman Sea by Eratosthenes (194–276 BC), a geographer from
Alexandria (Safiari, 2002). Nowadays, mangroves are found along
the Iranian beaches of the Persian Gulf and Oman Sea, as well as
around Bahrain, Qatar, Saudi Arabia, and the United Arab Emirates
(Bayoumi & El‐Nagar, 2009). Mangrove forests on the southern coast
of Iran are given the common title “The Hara forests,” and they cover
several locations between the 25°11′ and 27°52′ parallels longitude
Received: 9 March 2017
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Revised: 27 December 2018
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Accepted: 1 March 2019
DOI: 10.1111/maec.12544
REGULAR PAPER
A study of crude oil‐degrading bacteria from mangrove forests
in the Persian Gulf
Iman Fakhrzadegan
1
| Mehdi Hassanshahian
1
| Majid Askari Hesni
1
| Amir Saadatfar
2
1
Department of Biology, Faculty of
Science, Shahid Bahonar University of
Kerman, Kerman, Iran
2
Research and Technology Institute of
Plant Production (RTIPP), Shahid Bahonar
University of Kerman, Kerman, Iran
Correspondence
Mehdi Hassanshahian, Department of
Biology, Faculty of Science, Shahid Bahonar
University of Kerman, Kerman, Iran.
Email: mshahi@uk.ac.ir
Funding information
Shahid Bahonar University of Kerman
Abstract
Mangroves are coastal ecosystems, found in tropical and subtropical regions around
the world. They are found in the transitional zones between land, sea, and rivers.
Petroleum hydrocarbons are the most common environmental pollutants, and oil
spills pose a great hazard to mangroves forests. This research was focused on the
isolation and characterization of crude oil‐degrading bacteria from mangrove ecosys‐
tems at the Persian Gulf. Sixty‐one crude oil‐degrading bacteria were isolated from
mangrove samples (plant, sediment, and seawater) that enriched in ONR7a medium
with crude oil as only carbon source. Some screening tests such as growth at high
concentration of crude oil, bioemulsifier production, and surface hydrophobicity
were done to select the most efficient strains for crude oil degradation. Molecular
identification of strains was carried out by amplification of the 16S rRNA gene by
PCR. The results of this study were indicated that the quantity of crude oil‐degrading
bacteria was higher in the root of mangrove plants compare to other mangrove sam‐
ples (sediment and seawater). Also, identification results confirmed that these iso‐
lated strains belong to Vibrio sp. strain NW4, Idiomarina sp. strain BW32, Kangiella sp.
strain DP40, Marinobacter sp. strain DW44, Halomonas sp. strain BS53, and Vibrio sp.
strain DS35. The application of bioremediation strategies with these bacteria can
reduce crude oil pollution in this important marine environment.
KEYWORDS
bacteria, biodegradation, contamination, mangrove, Persian Gulf