34 International Journal of Research in Cosmetic Science 2013; 3(2): 34-39 ISSN 2277–7172 Original Article Screening of sun protective activity of fruit extracts of Garcinia indica. Mamata Dike 1 , Jayesh Anerao 2 , Renuka Thergaonkar 3 , Manjushree Deodhar *. Department of Biotechnology 1 , Department of Botany 2 , Department of Cosmetology 3 , Department of Botany * KET‟s V.G. Vaze College of Arts, Science and commerce, Mithagar road, Mulund (E), Mumbai 400081, India. Corresponding Author*: Dr. M. A. Deodhar. Tel: 9870176411 E-mail: drmadeodhar@gmail.com Received 25 September 2013; Accepted 12 October 2013 Abstract Risk of harmful effects due to exposure of UV radiations is increasing day by day, with increase in pollution, which causes depletion in ozone layer, thus UV protective products are becoming essential commodity of life. The chemical sun-blockers ZnO and TiO 2 provide broad spectrum UV protection, but their safety is doubted. Aim of this work was to study UV protective activity of fruit components of Garcinia indica. Ethanolic, Methanolic and Ethyl acetate extract of fruit rinds were screened for UV protective activity. Ethanolic and Methanolic extract showed absorbance in UV B and UV C region while ethyl acetate extract showed absorbance in broad range including UV A and UV B region. Dried Ethyl acetate extract of fruit rinds was compared with TiO 2 by using spectrophotometric method at various concentrations.UV protective activity shown by ethyl acetate extract was better than TiO 2 from 0.4 mgml -1 concentration and beyond. Ethyl acetate extract of kokum butter gave absorbance in UV B region. SPF of dried ethyl acetate extract and kokum butter was determined which were incorporated in sunscreen formulation. At 1.5% concentration of dried extract in sunscreen formulation showed very good SPF 2.02 with boot- star rating 5. Thus it can serve as an effective component for sunscreen products. © 2013 Universal Research Publications. All rights reserved Key words: UV protective activity, Ethyl acetate extract, SPF, Benzophenones 1. Introduction: Exposure to UV radiation has acute chronic and delayed effects on skin. UV radiation induced acute responses include inflammation such as sunburn, pigmentation, hyperplasia etc. Chronic effects include Photo-aging and photo- carcinogenesis [1] Solar UV radiations comprises of UV-C (200-280 nm), UV-B (280-320 nm) and UV-A (320-400 nm). UV-C is the most biologically damaging but it is filtered out by ozone layer while UV B and UV A radiations are the main cause of immunosuppression and photodamage. Skin care products for UV protection should be used to reduce harmful effects of UV radiations. The sunscreen products mainly include physical sunblockers like zinc oxide, Titanium dioxide; both provide broad spectrum UV protection. But they cause whitening phenomenon which seriously affects aesthetics. The organic sunscreen components include variety of chemicals like benzophenone, octyl methoxy cinnamate, alkylbenzoate, octylsalycilate mainly block UV B radiation [1].The chemical sunblockers like arobenzone has been reported to be unstable when combined with physical agents. It is necessary to prepare formulation which will have broad spectrum UV protection. In the quest to find effective photoprotective agent, plant derived products have been widely researched.In green tea or black tea tannic acid and theobromine help to remove heat from sunburns as well as catechins help prevent and repair skin damage. In red algae Porphyra UV absorbing amino acids like mycosporin show UV protective activity [2]. Other natural sources of antioxidants like anthocyanins, quercetin, apigenin, carotenoidetcare are also involved in UV protective activity [3]. Garcinia indica is an endemic fruit tree found in Western Ghats of India. Fruit rinds are rich source of anthocyanins Available online at http://www.urpjournals.com International Journal of Research in Cosmetic Science Universal Research Publications. All rights reserved