European Journal of Molecular & Clinical Medicine ISSN 2515-8260 Volume 7, Issue 2, 2020 2009 Assessment and comparison of Periostin levels in peri-implant sulcular fluid as a biomarker in healthy peri-implant sites and sites with peri- implant mucositis and peri-implantitis. Dr. Anup U Shelke, PhD Scholar, Department of Periodontics & Implantology, Sharad Pawar Dental College & Hospital, Datta Meghe Institute of Medical Sciences, Sawangi (M), Wardha. Email: dranup001@gmail.com Dr. Prasad V Dhadse, Professor & HOD, Department of Periodontics & Implantology, Sharad Pawar Dental College & Hospital, Datta Meghe Institute of Medical Sciences, Sawangi (M), Wardha. Email: drprasaddhadse@gmail.com Abstract: Background – Peri-implant diseases need early diagnosis and intervention in order to prevent implant failure. Periostin is an enzyme imperative for periodontal health which decreases with increasing severity of inflammation and levels revert back to normal after treatment. Alterations in the levels of periostin with relation to peri-implant diseases is not yet been investigated. This study aims to explore the role of periostin in peri-implant health and disease. Objectives: To evaluate and compare the levels of Periostin in peri implant sulcular fluid (PISF) in healthy peri-implant sites and sites with peri-implant mucositis and peri- implantitis with its clinical correlation. Method and design: Total of 66 implant sites loaded at least one year earlier will be included in this study and divided into three groups. Group I (healthy peri-implant tissues), group II (peri-implant mucositis), and group III (peri- implantitis). Levels of periostin in peri-implant sulcus fluid (PISF) acquired by micro- capillary pipettes acquired by micro-capillary pipettes will be determined from each group using human periostin ELISA kit. Results: The results will be analyzed as levels of periostin in peri-implant sulcular fluid recorded by ELISA in all the three groups and will be subjected to intergroup comparison and will be presented after statistical analysis. Conclusion: Levels of periostin in peri-implant sulcular fluid may prove to be a magnificent tool in early and prompt diagnosis of peri-implant diseases which will help in planning and execution of appropriate treatment, hence preventing further destruction of supporting tissues increasing longevity of dental implants. Keywords: Periostin, Peri-implant mucositis, Peri-implantitis, PISF Background and rationale: As with periodontal diseases, the biofilm is contemplated as the principal etiological factor for inception and advancement of destruction of peri-implant tissues and mediators of inflammation stimulated through microbial invasion are accountable for the development of peri- implant diseases such as peri-implant mucositis and peri-implantitis. 1,2