INTERNATIONAL JOURNAL OF LATEST TECHNOLOGY IN ENGINEERING, MANAGEMENT & APPLIED SCIENCE (IJLTEMAS) ISSN 2278-2540 | DOI: 10.51583/IJLTEMAS | Volume XII, Issue II, February 2023 www.ijltemas.in Page 1 Management of WSSV Challenge Test with Ig-Y with the Cohabitation Method Ferdi Fathurohman 1* , Nurul Mukminah 1 , Nurizzi Rifqi Ferdian 2 1 Agroindustry Study Program, Subang State Polytechnic, Subang, Indonesia 2 Machine Maintenance Study Program, Subang State Polytechnic, Subang, Indonesia * Corresponding author Abstract --This study aims to determine the effect and best dose of Ig-Y on WSSV in vannamei shrimp using the cohabitation method in the laboratory and in ponds. The method of this study was passively immunizing shrimp that had been infected with WSSV by immersing Ig-Y doses of 2% and 4% w/v. Statistical analysis using linear regression (T test and Determination Test). The results showed that the positive shrimp group had a mortality rate of 100% on the second day after the shrimp were infected with WSSV. The shrimp group treated with Ig-Y 4% had a mortality rate of 0%. While the shrimp group treated with Ig-Y 2% had a mortality rate of 0%, the morbidity rate reached 20% after contracting WSSV. The results of the T-test statistic test on giving Ig-Y 2% T-count value is greater than T-table (2.079 > 2.037 and Ig-Y 4% T-count value is greater than T-table (2.952 > 2.037) which means Ig -Y 2% and Ig-Y 4% had a positive effect on the healing of WSSV-infected shrimp, the results of the determination analysis of 2% Ig-Y and 4% Ig-Y were able to explain 58.4% of the variation in the shrimp recovery variable from WSSV infection, there was no significant difference between testing in the laboratory and in ponds. The conclusion of the WSSV challenge test with 2% Ig-Y and 4% Ig-Y using the cohabitation method on vannamei shrimp had a significant and positive effect on the recovery of vannamei shrimp. Keywords: Ig-Y, Cohabitation, Vannamei Shrimp, WSSV I. Introduction The Government of the Republic of Indonesia has regulated the Ministry of Maritime Affairs and Fisheries based on Presidential Regulation number 63 in 2015, it stated that marine and fisheries have given a positive impact to increase exports of fishery products which have so far been lagging compared to other agricultural sectors. One of the fishery products with high economic value is the Vannamei shrimp (Litopenaeus vannamei), therefore it is expected that the export target for Vannamei shrimp will occupy the main place in the list of exports of fishery products [1]. The demand for this commodity is always high even though there is often a decline in production. The imports result of fishery products in 2021 reached US$142.7 million or decreased by US$23 million compared to 2020 which reached US$165.7 million. Meanwhile, the import volume in 2021 was 145,227 tons or decreased compared to 2016 which amounted to 184,240 tons. Frozen fresh and processed shrimp experienced a decrease of 5% of the total imports of fishery products in 2021 [2]. The history of the emergence of the Vannamei shrimp farming business in Indonesia began with a decrease in the productivity of tiger shrimp (Penaeus monodon) which had triumphed as the flagship of Indonesian fishery exports. The decrease of the productivity is caused by disease, such as White Spot Syndrome Virus (WSSV). It is one of the main causes of the decline in tiger prawn cultivation in Indonesia. The shrimp farming business had slumped for several years, before finally entering Vannamei shrimp which were said to be free of viruses and diseases because they had passed a strict certification process. The strengths of Vannamei shrimp are not without flaws. Indonesian shrimp farmers are still dependent on other countries because Vannamei shrimp brooders that enter Indonesia must be imported from Hawaii (United States) as the center for the procurement of Vannamei shrimp seeds and brood stock. The mains exported to Indonesia are allegedly not the superior quality ones. As the result, the seeds productions have lower quality than those in their country of origin. Consequently, Vannamei shrimp production in Indonesia continues to decline. In addition, the existence of the White Spot Syndrome Virus (WSSV) also has decreased the productivity of Vannamei shrimp in Indonesia. Many studies have been conducted to make anti-virus and White Spot Syndrome Virus (WSSV) drugs. There have been research results from [3] and [4] using Ig-Y anti-WSSV by administering it intramuscularly to the shrimp's body. However, this method is less effective for large or massive scale. That is why, from this background, the researchers tried to conduct research to see the efficacy of Ig-Y as passive immunization of Vannamei shrimp against WSSV with the cohabitation method.