J Food Process Preserv. 2019;00:e14246. wileyonlinelibrary.com/journal/jfpp
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https://doi.org/10.1111/jfpp.14246
© 2019 Wiley Periodicals, Inc.
1 | INTRODUCTION
The common bean (Phaseolus vulgaris L.) is one of the most exploited
grain legume crops in Serbia. It is considered to be a crop with high
nutritional value due to its high content of proteins, dietary fibers, vita‐
mins, and minerals. Additionally, it is rich in many phytochemicals such
as polyphenols and lectins that have potential health benefits. Based
on the aforementioned facts the species Phaseolus vulgari is increas‐
ingly gaining attention as a component in functional foods (Pascale et
al., 2018). It possesses significant antiproliferative activity against colon
cancer (Compos‐Vega, Oomah, Loarca‐Piña, & Vergara‐Castañeda,
2013; Mentor‐Marcel et al., 2009) and prostate cancer (Kolonel et
al., 2000), as well as having antidiabetic potential (Mojica, Berhow, &
Gonzalez de Mejia, 2017). The two original backgrounds of common
bean are Middle American and Andean. This legume contributes to the
sustainable agriculture thanks to its ability to fix atmospheric nitrogen
(Schmutz et al., 2014). Common bean express a wide variety of grain
coat colors as one of the traits that define commercial types of com‐
mon bean. Genetic analyses have identified specific genes in charge
of grain coat color (P, C, R, J, D, G, B, V, and Rk) (McClean, Lee, Otto, &
Bassett, 2002). In common bean, the same group of genes that controls
Received: 3 January 2019
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Revised: 2 August 2019
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Accepted: 23 September 2019
DOI: 10.1111/jfpp.14246
ORIGINAL ARTICLE
Toward consistent discrimination of common bean (Phaseolus
vulgaris L.) based on grain coat color, phytochemical
composition, and antioxidant activity
Milica Ž. Karadžić Banjac | Strahinja Z. Kovačević | Aleksandra N. Tepić Horecki |
Zdravko M. Šumić | Anita S. Vakula | Sanja O. Podunavac‐Kuzmanović | Lidija R. Jevrić
Abbreviations: DPPH, 2,2‐diphenyl‐1‐picrylhydrazyl; MLR, multiple linear regression;
PCA, principal component analysis; TFC, total flavonoids content; TPC, total phenols
content; VIF, variance inflation factor.
Faculty of Technology Novi Sad, University
of Novi Sad, Novi Sad, Serbia
Correspondence
Strahinja Z. Kovačević, Faculty of
Technology Novi Sad, University of Novi
Sad, Bulevar cara Lazara 1, Novi Sad, Serbia.
Email: strahko@uns.ac.rs
Funding information
Ministry of Education, Science and
Technological Development of the Republic
of Serbia, Grant/Award Number: 31044 and
172012
Abstract
In this paper, 52 common bean (Phaseolus vulgaris L.) genotypes were analyzed re‐
garding their phytochemical composition and antioxidant activity, as well as other
characteristics (grain coat color, grain size, and growth size). The total phenols con‐
tent (TPC), determined according to the Folin–Ciocalteu method, varied from 0.166
to 0.772 mg/100 g DM, while total flavonoids content (TFC), determined by aluminum
chloride colorimetric assay, varied from 4.861 to 161.861 mg/100 g DM. Antioxidant
activity was assessed by 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) scavenging capacity
and ranged from 1.419 to 12.917 mg/ml. The antioxidant activity was predicted using
multiple linear regression (MLR). The obtained results revealed certain influence of
grain coat color on common bean phytochemical composition and antioxidant ac‐
tivity that was confirmed throughout classification method—principal component
analysis (PCA). White grain coat color samples were distinguished from colored ones
on the basis of their phytochemical composition and antioxidant activity.
Practical applications
The antioxidant activity of 52 common bean (Phaseolus vulgaris L.) genotypes was
predicted using MLR. A certain influence of grain coat color on common bean phy‐
tochemical composition and antioxidant activity was detected applying classification
chemometric techniques.