Anie et al Journal of Drug Delivery & Therapeutics. 2022; 12(5):64-69
ISSN: 2250-1177 [64] CODEN (USA): JDDTAO
Available online on 15.09.2022 at http://jddtonline.info
Journal of Drug Delivery and Therapeutics
Open Access to Pharmaceutical and Medical Research
Copyright © 2022 The Author(s): This is an open-access article distributed under the terms of the CC BY-NC 4.0 which
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Open Access Full Text Article Research Article
The Microbial Quality of Herbal Products
Anie, Oliseloke Clement
1
* , Egbon, Olukayode Temitope
2
, Enemchukwu, Chizoba Mercy
3
, Adushoke,
Eguonor Loveth
1
1. Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Delta State University, Nigeria.
2. Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Federal University, Oye-Ekiti. Nigeria.
3. Department of Pharmaceutical Microbiology and Biotechnology Faculty of Pharmacy Madonna University Elele Rivers State
Article Info:
_______________________________________________
Article History:
Received 17 July 2022
Reviewed 19 August 2022
Accepted 27August 2022
Published 15 Sep 2022
_______________________________________________
Cite this article as:
Anie OC, Egbon OT, Enemchukwu CM, Adushoke, EL,
The Microbial Quality of Herbal Products, Journal of
Drug Delivery and Therapeutics. 2022; 12(5):64-69
DOI: http://dx.doi.org/10.22270/jddt.v12i5.5590
_______________________________________________
*Address for Correspondence:
Anie, Oliseloke Clement, Department of
Pharmaceutical Microbiology and Biotechnology,
Faculty of Pharmacy, Delta State University, Nigeria.
Abstract
___________________________________________________________________________________________________________________
Background: Consumption of herbal products has increased dramatically during the last few
decades. Although there have been allegations that herbal products are made in unsanitary
conditions resulting in microbial contamination and health hazards consumed by patients.
Aim: The study is aimed at investigating the microbial quality of commonly sold herbal products in
Abraka, Delta State, Nigeria.
Methods: A total of seven (7) liquid herbal products (AB, GW, EC, IM, DR, GC and BM) were
randomly purchased from local herbal shops and pharmacy shops in Abraka. Samples were
aseptically measured out around the zones of a Bunsen fame and the total viable bacteria and fungi
counts of samples were determined using serial dilution and spread plate method.
Results: The total bacterial count in the herbal products ranged from 1.8 x 10
6
to 7.5 x 10
6
. Proteus
spp had the highest frequency of occurrence with a percentage of 50% while Staphylococcus spp.
occurred the least with a percentage of 10% while Candida spp. had the highest percentage
occurrence for fungi isolates and Aspergillus niger and Aspergillus flavus occurs less with percentage
occurrence of 10 and 20% respectively. The study also revealed that Tarivid, Streptomycin and
Gentamycin were susceptible to all bacterial isolated from the herbal products with average zone of
inhibition of 18.25±1.39, 19.75±3.39 and 23.13±2.0.8 respectively.
Conclusion: The finding of this study revealed that herbal products sold in Abrakawere
contaminated with microorganisms, some of which are pathogenic and thus, these herbal products
can serve as a means of spreading infections among the consumers.
Keywords: Herbal, microorganism, bacteria, Abraka.
INTRODUCTION
Herbal medicine is an ancient approach that is commonly used
in disease treatment. It is also known as complementary and
alternative medicine (CAM). It is growing increasingly popular
as breakthroughs in analysis and quality control, as well as
advances in clinical research, demonstrate the benefits of
herbal medicine in the treatment and prevention of diseases.
The term "herbal medications" refers to plants and plant
components that have been turned into phyto-
pharmaceuticals through simple harvesting, drying, and
storage methods.
1
Herbal remedies, according to the World
Health Organization, contain plant parts or plant material in
their raw or processed state as active components and may
contain excipients (foreign substances)
2
. Medicinal plant has
been defined by World Health Organization (WHO)
consultative group as any plant which in one or more of its
organs contains substances that can be used for therapeutic
purposes or which are precursors for the synthesis of useful
drugs.
3
It is essential to highlight that herbal medicines do not
include any combination of scientifically determined active
compounds or separated elements
4
. Globally, the usage of
herbal medicine is expanding.
5
. In poor nations such as Africa,
herbal medicine is used by up to 80% of the population as a
primary source of therapy.
5
Plants are rich in a wide variety of
secondary metabolites such as tannins alkaloids and
flavonoids, which have found in vitro to have antimicrobial
properties.
6
Although the World Health Organization (WHO)
has urged for the incorporation of herbal medical products
(HMPs) into developing nations' primary health care systems,
herbalists continue to neglect safety concerns with herbal
medication formulations. As a result, the safety of herbal
products has become a serious public health problem.
7
This is due to the fact that microorganisms of various types
are normally adherent to the leaves, stems, flowers, seeds, and
roots from which herbal medicine can be prepared, and
potential pathogens may also be introduced during harvesting,
handling, open-air drying, preserving, manufacturing
7
and the
use of contaminated materials for storage.
8
According to
certain findings, consumers may become unwell as a result of
ingesting herbs contaminated with pathogenic microbes.
9
and
the presence of antibiotic resistant microbial isolates in HMPs
may result in the transfer of antibiotic resistance strains to
consumers.
10
Herbal medicine and preparations have been widely used for
thousands of years in both developing and developed
countries due to their natural origins and lower negative