27th World Congress on Ultrasound in Obstetrics and Gynecology Short oral presentation abstracts Objectives: To assess quality of measurements of mean arterial pressure (MAP), mean uterine artery pulsatility index (UtPI), pregnancy-associated plasma protein A (PAPP-A) and placental growth factor (PlGF) in first trimester screening for pre-eclampsia program. Methods: Consecutive patients attending first trimester screening for aneuploidies in a large practice in Sydney, Australia, from May 2014 to February 2017 also had combined screening for pre-eclampsia based on the Fetal Medicine Foundation (FMF) algorithm. Distributions of MAP, UtPI, PAPP-A and PlGF, expressed as multiples of the median (MoM) were plotted in relation to the previously published UK median. Temporal analyses were produced by cumulative sum charts (CUSUM) and monthly distributions. Central tendency and dispersion charts were also produced for each individual UtPI operator. Results: 26,543 women attended for first trimester assessment and 21,010 had screening for pre-eclampsia with all four markers measured. Median MAP (n=26,450) UtPI (n=25,672), PAPP-A (n=23,471) and PlGF (n=21,723) MoM were 0.959, 1.031, 1.137 and 0.977, respectively. Of those, MAP and PAPP-A measurements were outside the expected range (±0.2 SD Log 10 [MoM]), and PlGF had a negative bias after March 2016. Most sonographers (40/46) measured UtPI within the acceptable range. The screen positive rate (SPR) for preterm pre-eclampsia, using a cut-off of 1:100, was 11.8%, similar with previous validation studies. Conclusions: While slight variations on biomarkers could be due to population characteristics and are unlikely to have major consequences on detection rate, bigger biases might affect significantly the SPR. Therefore, quality assurance process is essential in a first trimester screening program. OP17.07 Worsening of the uterine artery Doppler is associated with the development of hypertensive disorders of pregnancy J. Binder 3,2 , C.A. Monaghan 3,1 , S. Carta 3 , B. Thilaganathan 3 , A. Khalil 3 1 Fetal Medicine Department, Royal Maternity Hospital, Belfast, Co Down, United Kingdom; 2 Department of Obstetrics and Fetomaternal Medicine, Medical University of Vienna, Vienna, Austria; 3 Fetal Medicine Unit, St George’s Hospital Medical School, University of London, London, United Kingdom Objectives: Hypertensive disorders of pregnancy (HDP) are associ- ated with impaired placentation, as evidenced by abnormal uterine artery (UtA) Doppler. UtA mean pulsatility index (PI) shows a progressive decline with gestational age (GA). However, previous studies have reported that a proportion of pregnancies demonstrate worsening of the UtA Doppler. The aim of this study was to investi- gate the incidence of HDP according to the change in the UtA mean PI between the second and third trimester. Methods: This cohort study included singleton pregnancies under- going longitudinal UtA Doppler assessment in the second and third trimester. All the parameters were converted into centiles and multi- ples of the median (MoM) adjusting for GA. The study cohort was divided into 2 groups according to the change in the UtA Doppler between the second and third trimesters (decline or no change vs increase in the mean PI MoM). HDP included women who developed pre-eclampsia and gestational hypertension. Regression analysis was used to adjust for potential confounders. Results: The analysis included 5887 pregnancies. The incidence of HDP was significantly higher in the group with worsening of the UtA mean PI compared to those without (7.9% vs 5.8%; p<0.002). Logistic regression analysis demonstrated that both the second trimester UtA PI MoM (OR 8.12, 95% CI 5.07-13.00; p<0.001) and the difference between the 2 trimesters (OR 3.41, 95% CI 2.434-4.768; p<0.001) were significant independent predictors for the development of HDP. Conclusions: Worsening of the UtA Doppler, independent of the value recorded in the second trimester, is associated with HDP. OP17.08 Echocardiography and heart rate variability in pregnant women with essential arterial hypertension and pre-eclampsia V. Makukhina Kuban State Medical University, Krasnodar, Russian Federation Objectives: Pre-eclampsia is associated with pregnancy in 2-8% of cases. Arterial hypertension presents danger for the mother and fetus and provokes pre-eclampsia. Our research represents a comparative evaluation of echocardiog- raphy and heart rate variability (HRV) data of pregnant women with essential arterial hypertension, gravidas suffering from pre-eclampsia and healthy carriers. Methods: Three groups of pregnant women were examined: 11 had essential arterial hypertension (group 1, average age 35,3±1,0), 10 had pre-eclampsia (group 2, average age – 30,1±1,7) and 5 were healthy (group 3, 33±1,7 age). An echocardiography and daily ECG-monitoring with HRV evaluation were conducted for all patients. Results: The study has revealed a statistically significant increase in the interventricular septum thickness in the group 1, when compared to the group 2 (p<0.05). At the same time left ventricle (LV) ejection fraction was significantly higher in the group 2 than in other groups (p<0.05). There was no difference in the end-diastolic size and LV myocardium masse index between the groups. Results showed a statistically significant decrease of standard deviation normal to normal beat (SDNN) (p<0,01), SDNNi (SDNN index) and SDANNi (p<0,05) in group 1 compared with group 3. A strong negative correlation was found between the age of patients in the group 1 and their SDNN (correlation coefficient -0,8) and rMSSD data values (correlation coefficient -0,85). All pregnant women with obesity in groups 1 and 2 had different grade of uterine-placental hemodynamics disorders (odds ratio – 3,75). Conclusions: Carriers with pre-eclampsia have increased left ventricle ejection fraction, which is not accompanied with the decline of HRV measures. Pregnant women with essential arterial hypertension have deterioration of HRV measures. Obesity is associated with more frequent cases of uterine-placental hemodynamics disorders. OP17.09 Flow mediated dilation and peripheral arterial tonometry are disturbed in pre-eclampsia and reflect different aspects of endothelial function D. Mannaerts 1,2 , E. Faes 1,2 , E. Van Craenenbroeck 3,4 , C. De Bruyn 2 , M. Spaanderman 5 , W. Gyselaers 6 , Y. Jacquemyn 1,2 1 Research Group ASTARC, Antwerp Surgical Training, Anatomy and Research Centre, University of Antwerp, Antwerp, Belgium; 2 Obstetrics and Gynecology, Antwerp University Hospital, Antwerp, Belgium; 3 Laboratory for Cellular and Molecular Cardiology and Department of Cardiology, Antwerp University Hospital, Antwerp, Belgium; 4 Research Group Cardiovascular Diseases, Translational Pathophysiological Research, Antwerp University Hospital, Antwerp, Belgium; 5 Obstetrics and Gynecology, MUMC Maastricht University, Maastricht, Netherlands; 6 Obstetrics and Gynecology, Hasselt University, Genk, Belgium Objectives: Endothelial function and arterial stiffness are known to be altered in pre-eclamptic pregnancies. Previous studies have shown conflicting results regarding the best technique for assessing vascular © The Authors 2017 104 © Ultrasound in Obstetrics & Gynecology 2017; 50 (Suppl. 1): 48–153.