protocols.io | https://dx.doi.org/10.17504/protocols.io.bp2l691q5lqe/v1 May 24, 2023 1/5 May 24, 2023 Isolation, activation, and retroviral transduction of primary T cells from murine splenocytes In 1 collection DOI dx.doi.org/10.17504/protocols.io.bp2l691q5lqe/v1 Diana Rose E Ranoa , Preeti Sharma , Claire P. Schane , Amber N Lewis , Amber N Lewis , Edward Valdez , Edward J. Roy , David M. Kranz University of Illinois at Urbana-Champaign Diana Rose E Ranoa University of Illinois at Urbana-Champaign 1 1 1 1 1 1 1 1 1 DOI: dx.doi.org/10.17504/protocols.io.bp2l691q5lqe/v1 Protocol Citation: Diana Rose E Ranoa, Preeti Sharma, Claire P. Schane, Amber N Lewis, Amber N Lewis, Edward Valdez, Edward J. Roy, David M. Kranz 2023. Isolation, activation, and retroviral transduction of primary T cells from murine splenocytes. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l691q5lqe/v1 License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Protocol status: Working Created: October 28, 2022 Last Modified: May 24, 2023 Protocol Integer ID: 71948 Abstract This protocol outlines the steps for retroviral transduction of activated primary T cells isolated from spleen of donor mice.