~ 1 ~ Journal of Advances in Microbiology Research 2021; 2(2): 01-04 E-ISSN: 2709-944X P-ISSN: 2709-9431 JRM 2021; 2(2): 01-04 © 2021 JRM www.microbiojournal.com Received: 01-05-2021 Accepted: 06-06-2021 Emoleila Itoandon Department of Research and Development, Almond Research Foundation, Chennai, Tamil Nadu, India Femi Adams Production Analytical and Laboratory Management Department, Federal Institute of Industrial Research, Nigeria Tijani Azeez Production Analytical and Laboratory Management Department, Federal Institute of Industrial Research, Nigeria Correspondence Emoleila Itoandon Department of Research and Development, Almond Research Foundation, Chennai, Tamil Nadu, India Surface spreading sporulation (SSS) technique for microfungal purification Emoleila Itoandon, Femi Adams and Tijani Azeez DOI: http://dx.doi.org/10.22271/micro.2021.v2.i2a.13 Abstract A technique was used for the purpose of separating microscopic filamentous molds. The primary aim was to establish a method that will conveniently distribute vegetative spores across the surface of a culture medium allowing easy detection of desired strain. A contaminated Sabouraud Dextrose Agar (SDA) cultured plate previously used for isolation at 25 o C after 5 days was used for the purpose of this investigation. The culture plate showed a mixture of probable isolates after incubation period signifying a case of cross contamination. Following standard laboratory procedure, the following isolates: Aspergillus Niger, Aspergillus terreus, Penicillium funiculosum and Penicillium chrysogenum were recovered using the surface spreading technique. The investigation confirmed the level of microbial co-existence which is often difficult to manage in cases of pollution caused by molds due to their growth pattern. On the other hand, the practise provided a simple and effective application in handling purification of multicellular sporulation. Keywords: nutrients, cross contamination, purification, molds Introduction Green computing is the study in which discarding, recycling and building of computers and Molds are microscopic multicellular filamentous fungi with unique structural and functional properties. Their growth allow direct contact with the environment and based on different classification they form various types of effective nutritional habits enabling them cover a large surface area (Sun et al. 2019) [15] . These characteristics make diffusion of nutrient easy enabling the mold adapt to dehydration. Research activities have shown industrial relevance which has been justified in the proffering of enzymes, primary and secondary metabolites (Zhou et al. 2021; Suurbaar et al. 2017, Sunesen and Stahnke, 2003) [22, 17, 16] making them very significant to industrialization. Their ability to grow in extreme conditions has been related to the protective structured vegetative spores, which provide high level of modifications for survival (Selbmann et al. 2005) [14] . Spores of molds have been recorded to be small in sizes and easily propagated by wind, rain, insects, animals, and man constituting frequent level of cross contamination (Maharachchikumbura et al. 2016) [9] . Furthermore, with relevance to their sizes and growth pattern; purification for the purpose of strain identification is often a challenge because: i. their filaments often easily growing interwoven forming a web across any environmental surface and ii. Undetected vegetative spores passing through filtration medium causing progressing contamination. Several techniques have been used for the purpose of micro- fungi purification and aligned to methods of isolation from samples with common principles involving: i. single spore isolation method often employed when the fungus produces spores, which are coloured and bold, and ii. Single hyphal tip method, often used when purifying fungi which either do not produce spores or produces small and hyaline spores (Fawzi, 2011) [2] . Either way, purification still remains a challenge resulting to consistent environmental pollution. The main objective of this investigation was to develop a simple and cost effective technique which overcomes the selection between spore or hyphae producers, and also reduce the time involved in the process of mold identification. Materials and Methods Specimen Collection A contaminated culture plate was retrieved from a research institute located at longitude: 77°10′2.78′′E (77.167441) and latitude: 28°31′43.82′′N (28.52884) in the Northern part of India.