TL.13.002 Research On Paclitaxel-Carrying Nanometre Microbubbles Preparation and Characteristics Qiao Wang, J. I. Xiaojuan, Jingyu Chen, Ruling Yang, Jun Guo Children’s Hospital of Chongqing Medical University, Chongqing, China Brief Description of the Purpose of the Study: To prepare paclitaxel- carrying nanometre microbubbles(PNM) modified with human serum albumin(HSA) and study the Characteristics. Methods: The HSA modified PNM was prepared by thin-film dispersion method and mechanical vibration, the appearance,concentra- tion,mean diameter,zeta potential and the entrapment efficiency were detected. The normal rabbit’s iliac artery contrast imaging was observed. Main Results: This modified PNM had a good shape. The mean diam- eter was(772.9 6 6.2)nm, the concentration of bubble was (8.64 6 1.38)3109/ml, zeta potenital was(-13.2 6 0.3)mv, the entrapment effi- ciency of the modified bubble was (93.51 6 2.07)%, which was higher than that of no modified bubble (84.88 6 4.14)%(p , 0.05). It could make the rabbit iliac artery enhancement. Importance of the Conclusions: The HSA modified PNM was stable and effective, with smaller size and higher encapsulation efficiency. TL.13.003 Electrocardiogram Monitoring of Microlesion Induction by Contrast-Enhanced Pulsed Ultrasound for Therapeutic Myocardial Reduction D. L. Miller, C. Dou, G. E. Owens, O. D. Kripfgans University of Michigan, Ann Arbor MI, USA Brief Description of the Purpose of the Study: Myocardial contrast echocardiography can induce premature electrocardiogram complexes that are associated with myocardial microlesions. The objective was to assess the feasibility of utilizing this bioeffect for therapeutic myocar- dial reduction. Methods: The hearts of anesthetized rats were treated with 1.5 MHz ultrasound pulses in the presence of contrast-agent, guided by 8 MHz imaging. Five cycle pulses in eight pulse bursts were triggered at 1:4 heartbeats for 5 min. ECGs were monitored for premature complexes (PCs) to assess the rate and severity of induced cardiac injury, and Evans blue dye was used to indicate microlesions in 1 day samples. Main Results: The percentage of triggers inducing PCs was 0 in shams, 41 6 11 at 2 MPa rarefactional pressure (p , 0.02) and 68 6 13 at 4 MPa (p , 0.02). In addition, the rate of PCs declined significantly with time at 2 MPa, but not at 4 MPa, suggesting that the saturation effect seen for diagnostic ultrasound can be avoided in therapy. Evans blue staining showed scattered microlesions that corroborated the ECG indications of tissue injury. Importance of the Conclusions: Ultrasonic induction of microlesions can produce measured tissue reduction, which may be a more favorable method, than, for example, alcohol septal ablation, for the treatment of hypertrophic cardiomyopathy. TL.13.005 Which Elastographic Method Is More Feasible for Non-Invasive Evaluation of Liver Fibrosis? I. Sporea, S. Bota, A. Jurchis, O. Gradinaru-Tascau, A. Popescu, R. Sirli Department of Gastroenterology and Hepatology, -Victor Babes- University of Medicine and Pharmacy TimiOara, Romania Brief Description of the Purpose of the Study: Ultrasound based elas- tographic methods are increasingly used in clinical practice for liver fibrosis evaluation. The aim of our study was to assess the feasibility ("intend to diagnose") of the 3 shear waves elastographic methods (Transient Elastography-TE, Acoustic Radiation Force Impulse-ARFI and SuperSonic Shear Imaging-SSI). Methods: Our study included 332 subjects, with or without hepatopa- thies, in which liver stiffness (LS) was evaluated by means of TE, ARFI and SSI. Reliable measurements were defined as: median value of 10 (TE, ARFI) or 5 (SSI) LS measurements with a success rate$60% and an interquartile range interval,30%, values expressed in kPa (TE, SSI) or m/s (ARFI). Main Results: Reliable LS measurements were obtained in a signifi- cantly higher percentage of subjects by means of ARFI elastography as compared with TE and SSI: 92.1% vs. 72.2%, (p , 0.0001) and 92.1% vs.71.3%, (p , 0.0001), respectively. The rate of reliable LS measurements was similar for TE and SSI: 72.2% vs. 71.3%, (p 5 0.86). For both TE and SSI, older age and higher BMI were associated with the impossibility to obtain reliable measurements, while the pres- ence of chronic hepatopathies influenced the rate of reliable measure- ments only for SSI. For ARFI, no factor was identified to be associated with the impossibility to obtain reliable measurements. Importance of the Conclusions: The most feasible shear-waves ultra- sound elastographic method is ARFI. TL.13.006 Is Supersonic Shear Imaging (SSI) a Reliable Method for Liver Fibrosis Evaluation? I. Sporea, A. Popescu, S. Bota, A. Jurchis, O. Gradinaru-Tascau, R. Sirli Department of Gastroenterology and Hepatology, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania Brief Description of the Purpose of the Study: Aim: to evaluate if SSI is a reliable tool for liver fibrosis evaluation, considering Transient Elas- tography (TE) as the reference method. Methods: 332 subjects were evaluated by means of TE and SSI. Reli- able measurements were defined as: median value of 10(TE) or 5(SSI) LS measurements with a success rate$60% and an interquartile range interval,30%, values expressed in kPa. To discriminate between various stages of fibrosis by TE we used the liver stiffness (LS) cut- offs (kPa) proposed in the most recently published meta-analysis (Tso- chatzis, J Hepatol 2011): F1-6, F2-7.2, F3-9.6 and F4-14.5. Main Results: Our subjects were: healthy volunteers-16.9%; patients with chronic hepatitis B and C -44.8%;with non-viral chronic hepatopa- thies-27.4%; and with liver cirrhosis-10.9%. The rate of reliable LS measurements was similar for TE and SSI: 72.2% vs. 71.3%,p 5 0.86. Reliable LS measurements by both elastographic methods were obtained in 55.4% of patients. The distribution of liver fibrosis in this cohort of patients, using TE prespecified cut-off values were: F0- 40.2%, F1-13.1%, F2-20.1%, F3-14.1%, F4-12.5%. The AUROC of SSI for predicting F$1, F$2, F$3 and F54 were: 0.818, 0.851, 0.889 and 0.910, while the SSI cut-off values (kPa) were: 7.1, 7.8, 8.6 and 11.5. Importance of the Conclusions: SSI is a reliable method for non- invasive evaluation of liver fibrosis. TL.13.007 Usefulness and Limitations of the VirtuTRAX TM Instrument Navigator in Assisting RFA: A Pilot Study H. Tanaka, N. Ikeda, T. Takashima, K. Iwata, K. You, A. Ishii, N. Aizawa, Y. Sakai, Y. Iwata, H. Enomoto, M. Saito, S. Nishiguchi, H. Iijima Hyogo College of Medicine, Nishinomiya, Japan Brief Description of the Purpose of the Study: The VirtuTRAX TM Instrument Navigator is an exciting new advancement, which uses S64 Ultrasound in Medicine and Biology Volume 39, Number 5S, 2013