147| VOLUME 78 ISSUE 2 | NOVEMBER Sequence Analysis of the Internal Transcribed Spacer (ITS) Region of the Nuclear Ribosomal DNA (nrDNA), and trnL Intron of Chloroplast DNA (cpDNA) of the Chrysochamela (Fenzl) Boiss. (Brassicaceae) In Turkey Emre SEVİNDİK 1* and Mehmet Yavuz PAKSOY 2 1 Aydın Adnan Menderes University, Faculty of Agriculture, Department of Agricultural Biotechnology, South Campus, Cakmar, Aydin, Turkey 2 Munzur University, Tunceli Vocational School, Department of Medical Services and Techniques, Medical Documentation and Secretaryship Pr. Tunceli, Turkey * Corresponding author: E.Sevindik e-mail: ph.d-emre@hotmail.com RESEARCH ARTICLE Abstract Brassicaceae family is an important one since it includes many economic and significant industrial oilseeds, spices, vegetables and some forage plants. In this study, sequences analysis among Chrysochamela (Brassicaceae) species distributed in Turkey were conducted nrDNA ITS and cpDNA trnL intron sequences. Chrysochamela species were collected and brought to the laboratory. ITS and trnL intron sequences were corrected with BioEdit and FinchTV programs. As a result of the study, ITS nucleotide compound compositions were determined as 22.7% T, 29.1 C, 21.5% A and 26.7% G. The lowest distance was 0.000 and the highest distance was 0.037. The phylogenetic tree obtained using the MEGA 6.0 program consists of two large groups. According to trnL intron sequences 37.9% T, 18.4 C, 28.3% A and 15.5% G. Nucleotide compound compositions were determined. The genetic distance between species was determined between 0.000 and 0.022. Maximum likelihood phylogenetic tree consists of two large groups. As a result, phylogenetic analyzes using ITS and trnL intron sequences were compatible with each other. It was also in past studies found to be supported by morphological, anatomical and RAPD data. Keywords: Chrysochamela; ITS; trnL intron. INTRODUCTION Turkey, the origin of a number of crop plants and many species of plant and / or one of the centers of diversity (Balkaya and Karaagaç, 2006). Different ecological conditions have enabled Turkey to have such a great genetic (Pinar et al., 2021). The Brassicaceae comprises 49 tribes, 321 genera and 3660 species in the world (Al-Shehbaz, 2012). This family, in Turkey, consists of 88 genera and 539 species (Başer et al., 2018). This family includes many vegetable crops of the genera Brassica L. and Raphanus, L. sources of spices (Eutrema R.Br. and Armoracia Gaertn. Mey Et Schreb.) and vegetable oils (Brassica), ornamental plants (Arabis,L., Hesperis L., Lobularia Desv. and Matthiola R.Br.), and model species in experimental biology (for example, Arabidopsis thaliana (L) Heynhold) (Koch and Marhold, 2012; Guo et al., 2017; Esmailbegi et al., 2018). The genus Chrysochamela (Fenzl) Boiss., is one of the genus of Brassicaceae. In the flora of Turkey, there are 3 species of Chrysochamela genus viz., (Chrysochamela elliptica Received: 20 June 2021 Accepted: 14 September 2021 Published: 15 November 2021 DOI: 15835/buasvmcn-hort:2021.0006 © 2021 Authors. The papers published in this journal are licensed under the Creative Commons Attribution- NonCommercial-NoDerivatives 4.0 International License