Online First Article Comparative Effects of Deworming, Mineral and Vitamin Supplementation on Humoral Response against Peste de Petits Ruminants Vaccine in Goats Abdullah Iqbal 1 , Muhammad Abubakar 2 , Shumaila Manzoor 3 , Muhammad Kamran Ameen 4 and Rani Faryal 1 * 1 Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan. 2 National Veterinary Laboratory, Park Road, Islamabad, Pakistan. 3 Progressive Control of Peste des Petits Ruminants (PPR) in Pakistan, FAO, Islamabad. 4 Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan Article Information Received 23 July 2017 Revised 02 October 2020 Accepted 09 November 2020 Available online 08 June 2022 (early access) Authors’ Contribution RF and MA conceived the idea. AI, MKA and SM conducted the experiment and all authors participated in write up and approved the manuscript. Key words Peste des petits ruminants, Vaccination, Humoral immunity, Vitamins, Minerals This study aimed to evaluate the effect of mineral supplementation, vitamin supplementation and deworming on humoral response against PPR vaccine in local goats. Group 1 and group 2 goats were given mineral supplementation and fat-soluble vitamin supplementation for 21 days. Group 3 goats were twice dewormed with Fenbendazole. Group 4 bucks were kept as controls. All bucks were vaccinated with the Pestivac vaccine. Antibodies detection was done using competitive ELISA from serum samples. Antigen shedding by vaccinated animals via nasal and fecal route was checked by the haemagglutination test and RT-PCR. All group 2 bucks, developed humoral protection against PPR within the 1 st week. Only, 50 percent bucks of group 1 developed humoral protection against PPR after one week of vaccination. The mean percent inhibition value of competition-ELISA of group 2 was half the mean percent inhibition values of group 3 and group 4 after one week of vaccination. There was no antigen shedding through nasal or fecal route after vaccination in any animal included in the study. P este des Petits Ruminants (PPR) is a major viral disease of domesticated and wild small ruminants caused by Peste des Petits Ruminants Virus (PPRV). The first outbreak of PPR was reported from Pakistan back in 1994. Since, then many PPR outbreaks have been reported from various areas of Pakistan (Zahur et al., 2014). In the naïve population, morbidity and mortality rates of PPR can reach up to 80-100%, but morbidity and mortality rates are generally lower in endemic areas of Pakistan (Abubakar et al., 2008). Phylogenetic analysis of PPRV isolated from * Corresponding author: ranifaryal@qau.edu.pk 0030-9923/2022/0001-0001 $ 9.00/0 Copyright 2022 by the authors. Licensee Zoological Society of Pakistan. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). outbreaks in Pakistan indicated that PPRV present in Pakistan belongs to lineage IV of PPRV (Munir et al., 2011). Live attenuated vaccine of PPRV Nigerian strain 75/1 is commonly used in Pakistan (Abubakar et al., 2015). Minerals and vitamins play a significant role in the development of both innate and adaptive immune responses. Endoparasites use the nutrients of animals and cause stress on animals (Hanssen et al., 2013). The present study was designed to evaluate and compare the effect of mineral supplementation, vitamin supplementation and deworming on the development of antibody-mediated immune response against PPR vaccine in the local goat breed to improve health for better meat production. Material and methods A total of 32 bucks having the same age and sero- negative for PPR were selected for this study. All experimental animals were injected with 1 ml of Pestivac Jovac, (Jordan) live attenuated PPR vaccine via the subcutaneous route with a separate disposable syringe. ABSTRACT Pakistan J. Zool., pp 1-3, 2022. DOI: https://dx.doi.org/10.17582/journal.pjz/20170723160722 Short Communication