Journal of Progressive Research in Biology (JPRB) ISSN 2454-1672 Volume 3, Issue 1 available at www.scitecresearch.com/journals/index.php/jprb 119 SCITECH Volume 3, Issue 1 RESEARCH ORGANISATION Published online on August 25, 2016 Journal of Progressive Research in Biology www.scitecresearch.com/journals Exploration of Active Metabolites from Aegle Marmelos and Emblica Officinalis for Determining its Immunosuppressive Properties Amit Gupta,* Ajam C Shaikh and Sushama R Chaphalkar Department of Immunology and Virology, Vidya Pratishthan’s School of Biotechnology (VSBT, Research Centre affiliated to Savitribai Phule Pune University), Baramati-413133, Maharashtra, India. * Corresponding author Dr Amit Gupta Assistant Professor/Senior Scientist 1. Abstract As per the literature, bioactive compounds isolated or purified from medicinal plant products that are generally used for curing various human diseases. The present study involves two different medicinal plants i.e. Aegle marmelosand Emblica officinalis were locally available in Baramati region of Maharashtra, India. The leaves of these medicinal plants were used for phytochemical analysis in aqueous extract using phosphate buffered saline (PBS; pH 7.2) in order to find out the active metabolites using liquid chromatography mass spectrometry (LC-MS). For these studies, aqueous leaves extract of these two medicinally plants were tested for immunological studies in mice model studies especially Swiss mice pertaining to determine splenocyte proliferation assay using hepatitis B vaccine containing surface antigen (HBsAg) and also determined pro- inflammatory cytokines (IFN-gamma and TNF alpha) by Elisa. The results showed that aqueous leaves extract showed declined in splenocyte proliferation assay and proinflammatory cytokines (FN-gamma and TNF alpha) at higher doses as compared to control samples. HBsAg (20 μg/ml) used as standard for these studies. Overall, the results showed that aqueous leaves extract of Aegle marmelosand Emblica officinalis showed immunosuppressive activity against HBsAg as compared to control. Keywords: Aegle Marmelos; Emblica Officinalis; Proinflammatory; Hepatitis B Vaccine. 2. Introduction India has a rich history of using various medicinal plant products for treating various diseases. Most of the medicinal plants are investigated or reported for its beneficial use against different types of diseases and reported as well as published in numerous scientific journals [1, 2]. In other words, use of medicinal plant products as a source of medicine against human diseases and showed its importance in human health care system in India [3]. Currently, most of the medicinal plants are recommended for various diseases having no side effects. As per Indian traditional system, these medicinal plants are continuously used for therapy related to cardiovascular disease and inflammation [4, 5].Several medications that are available to suppress over-reactive T lymphocytes, but many of the currently marketed drugs produce severe and life-threatening side-effects [6]. The most commonly used immunosuppressive drugs especially Cyclophosphamide, betamethasone etc. and historically one of the oldest are the glucocorticoids [6, 7]. These immunosuppressive drugs are generally restricted and inhibited the synthesis and secretion of inflammatory cytokines and support the activation of anti-inflammatory signalling cascades. In addition, these immunosuppressive drugs showed various side effects including toxic effect as well [6, 7]. However, many of immunosuppressive drugs cause unwanted and severe side-effects in patients and therefore there is a high demand for medicinal plant products that showed less- toxic immunosuppressive pharmaceuticals [8, 9].