© 2024 IJRAR February 2024, Volume 11, Issue 1 www.ijrar.org (E-ISSN 2348-1269, P- ISSN 2349-5138) IJRAR24A1933 International Journal of Research and Analytical Reviews (IJRAR) 314 PROFILE ASSESSMENT OF VIT E ROYAL JAYANTILAL PADARIYA 1* , VIJAYKUMAR BODARYA 2 1 Department of Chemistry, Fergusson College, University of Pune – 411007, India. Department of Physics, Sardar Patel University, Vallabh Vidhyanagar – 388120, India. ABSTRACT A reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the determination of vitamin E in food and pharmaceutical samples. The method was based on the separation of vitamin E from other interfering compounds using a C18 column and a mobile phase of acetonitrile:methanol (75:25). The detection wavelength was 220 nm. The method was linear over the concentration range of 1-200 μg/mL, with a correlation coefficient of 0.9999. The intra-day and inter- day precisions were 0.5% and 1.0%, respectively.[12] The accuracy of the method was determined by spiking standard solutions into blank samples and the recovery was found to be 98.5%. The method was successfully applied to the determination of vitamin E in a variety of food and pharmaceutical samples.[7,2,13] Stainless steel C18 columns was used as a chromatographic column, d=250mm, phi=4.6mm, 5μ. As a mobile phase, a methanol: water (98:2) mixture was used.[15] The detection was performed at a wave length of lambda=290 nm for a 20 uL injection volume with a flow rate of 0.6mL/min. The method was applied with good results in determining the vitamin E content in different type of finish products and it was validated. The proposed method is quick, reproducible and accurate, being characterized by a obtained results.[6,17] KEYWORDS: RP-HPLC, vitamin E, tocopherols, tocotrienols, mass spectrometry. INTRODUCTION Sophisticated analytical methods such as high-performance liquid chromatography (HPLC) may be used to distinguish, recognise, and measure an extensive range of substances. It works especially well for complicated combination analysis, including those of pharmaceutical and food samples.[1,2]. The development of an HPLC method for vitamin E involves a number of steps, including: Selection of the appropriate column and mobile phase, Optimization of the elution conditions, Determination of the detection wavelength and Validation of the method. The following factors should be considered when selecting the column and mobile phase, the polarity of the vitamin E compounds,[21,23] the sample's other interfering substances and the intended separation of the various vitamin E isomers.[23] The elution conditions can be optimized by varying the composition of the mobile phase, the flow rate, and the column temperature. The detection wavelength should be selected based on the absorbance spectra of the vitamin E compounds.[4,10,14] The method should be validated to ensure that it is accurate, precise, and reproducible. This involves analyzing a series of standard solutions and blank samples[3,7,11]. The selection of the appropriate HPLC column and mobile phase is important for achieving a good separation of vitamin A from other compounds in the sample. The mobile phase should be a mixture of organic solvents that are miscible with water. The column should be a reversed-phase column, which means that The mobile phase is hydrophilic, whereas the stationary phase is hydrophobic.[3,15]. The optimization of the separation conditions involves adjusting the flow rate, the temperature of the column and the mobile phase's makeup. The goal is to achieve a separation that is sharp and reproducible.[16]