Chemistry Volume 14 Issue 1 Version 1.0 Year 2014 Type : Double Blind Peer Reviewed International Research Journal Publisher: Global Journals Inc. (USA) Online ISSN: 2249-4626 & Print ISSN: 0975-5896 Analysis by GC-MS of an Aza-Michael Reaction Catalyzed by CALB on an Orbital Shaker and under Microwave Irradiation By Sandra S. Ribeiro, Marciana P. Uliana, Timothy J. Brocksom & André L. M. Porto Universidade De São Paulo, Brazil Abstract- In this study, aza-Michael reactions between 1- phenylmethanamine and α,β-unsaturated cyclohexenones were investigated, using lipase from Candida antarctica. The reactions were performed in various organic solvents (CH 2 Cl 2 , hexane, MeOH, toluene, THF) under mild conditions, with orbital shaking and microwave irradiation. The reactions showed good results in the presence of CALB, yielding the Michael adducts and imines. The reaction products were analyzed by GC-MS and in some cases it was found that the reverse aza-Michael reaction had occurred. GJSFR-B Classification : AnalysisbyGC-MSofanAza-MichaelReactionCatalyzedbyCALBonanOrbitalhakerandunderMicrowaveIrradiation Strictly as per the compliance and regulations of : © 2014 Sandra S. Ribeiro, Marciana P. Uliana, Timothy J. Brocksom & André L. M. Porto. This is a research/review paper, distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License http://creativecommons. org/ licenses/by-nc/3.0/), permitting all non commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. FOR Code: 100505, 250106 Global Journal of Science Frontier Research: B Keywords: biocatalysis; amines; aza-Michael addition; enones; imines.