_____________________________________________________________________________________________________ *Corresponding author: E-mail: alex.ogregedi@gmail.com; International Research Journal of Gastroenterology and Hepatology 3(4): 13-24, 2020; Article no.IRJGH.61469 Hepatoprotective Effects of Hypoestes rosea in Acetaminophen-Induced Toxicity in Albino Rats Ogregade, I. E. 1* , Igwe, F. 2 and Davies, T. G. 1 and Bartimaeus, E. S. 1 1 Department of Medical Laboratory Science, Rivers State University, Port Harcourt, Nigeria. 2 Department of Biochemistry, Rivers State University, Port Harcourt, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. Author SBE designed the study. Author IF wrote the protocol. Author GDT wrote the first draft of the manuscript. Author EOI managed the analyses of the study and the literature searches. All authors read and approved the final manuscript. Article Information Editor(s): (1) Dr. Juan Carlos Martín del Olmo, Medina del Campo Hospital, Spain. Reviewers: (1) Shree Lakshmi Devi.S, Shri Sathya Sai Medical College and Research Institute, India. (2) Shweta Anand, Sardar Vallabhbhai Patel University of Agriculture & Technology (SVPUA&T), India. Complete Peer review History: http://www.sdiarticle4.com/review-history/61469 Received 17 July 2020 Accepted 23 September 2020 Published 01 October 2020 ABSTRACT Aims: The aim of this study was to evaluate the hepatoprotective effects of Hypoestes rosea in acetaminophen-induced toxicity in albino rats. Study Design: This study is a case-controlled interventional study. Place and Duration of Study: This study was conducted at the Experimental Animal Unit of the Department of Human Physiology, University of Port- Harcourt, between June 2018 and December, 2019. Methodology: A total of 112 adult apparently healthy albino rats weighing (180-220 g) were used for this study, the rats were divided into six experimental groups of extract control (EC), negative control (NC), positive control (PC), aqueous extract of Hypoestes rosea (AEHr) 100 mg/kg body weight (b w), AEHr 200 mg/kg b w., and AEHr 300 mg/kg b w. groups each of six rats. The study groups comprised of two treatment phases each, (Pre-treatment and Post-treatment phases), duration of treatment (Acute and Sub-chronic) with six experimental groups in each of the phases. At the end of the study period, blood sample were taken through jugular vein under chloroform anaesthesia in a desiccator for liver function parameters Total Bilirubin (TB), Conjugated Bilirubin (CB), Aspartate aminotransaminase (AST), Alanine aminotransaminase (ALT), Alkaline Original Research Article