Constructed wetlands for treatment of harvest effluents from grow-out ponds of the Amazon river prawn Alexandre Augusto Oliveira Santos 1,2 & Antonio Fernando Monteiro Camargo 1,2 1 CAUNESP UNESP, Aquaculture Center, Jaboticabal, Brazil 2 Departament of Ecology, Institute of Biosciences, UNESP, Rio Claro, Brazil Correspondence: A F M Camargo, CAUNESP UNESP, Aquaculture Center, Prof. Paulo Donato Castellane, 14.884-900 Jabotica- bal, SP, Brazil. E-mail: afmc@rc.unesp.br Abstract Effluent discharges from aquaculture can reduce water quality in receiving water bodies and that strategies or practices to reduce this are necessary. One possibility is to reduce, or eliminate, water renewal in grow-out ponds. In this study, we elimi- nated water renewal in grow-out ponds associated with the culture of 40 individuals m 2 of Amazon river prawn (Macrobrachium amazonicum). At the end of the culture period it was, however, necessary to drain the pond to harvest the prawns. An experi- ment was performed in triplicate, in which the water supply characteristics and harvest water characteristics of ponds were evaluated. To reduce these concentrations of total N and P, an aquatic macrophyte (Eichhornia crassipes, water hyacinth) treatment system (CWs) was adopted. The water characteristics in the CWs were evaluated after 1, 3, 7, 14 and 21 days. The water supply of ponds presented the average concentrations of 0.67 0.32 mg L 1 and 17.4 14.7 lgL 1 of total-N and total-P respectively. The harvest effluent of ponds had elevated concentrations of different forms of nitrogen (4.44 mg L 1 of total-N) and phosphorous (100.9 lgL 1 of total-P). After 1 day of the experiment we found the following reductions in key nutrients in treatment system containing E. crassipes: 90%, 78% and 45% reductions in the concentrations of particulate matter, orthophos- phates and nitrates respectively. We noted that after 3 days the nitrates had been reduced by 53%. We concluded that 3 days of this treatment was suffi- cient for the removal of the additional nutrients that had accumulated in the Amazon river prawn ponds. Keywords: CWs, removal efficiency, Macrob- rachium amazonicum, Eichhornia crassipes, macro- phytes, water hyacinth Introduction The farming of aquatic organisms, including shrimp, provides social and economic benefits, but has raised concerns relating to associated environ- mental impacts (Goldburg & Triplett 1997). Only 2122% of nitrogen and 6% of phosphorus sup- plied in the diet are assimilated by the shrimp (Jackson, Preston, Burford & Thompson 2003). Thus, the majority of nitrogen and phosphorus remains, in a dissolved or particulate form, in the water or in sediments, or becomes incorporated into other organisms. Aquaculture effluents pro- duced by feed or discharged by various animals into natural waters can have a number of negative impacts on the environment (Cho & Bureau 2001), including the promotion of eutrophication. To pre- vent eutrophication, several alternatives can be considered, such as effluent treatment to remove nutrients and particulate matter from aquaculture effluents. The use of constructed wetlands (CWs) to treat aquaculture effluents is a solution that has been recently used (Henry-Silva & Camargo 2006, 2008). This system has a number of advantages: it is a natural process, has high process stability, and it is cost-effective. Furthermore, CWs are relatively simple to build and operate, which is particularly desirable in developing countries (Trang, Konn- erup, Schierup, Chiem, Tuan & Brix 2010). The use of aquatic macrophytes for water treatment has been shown to be very efficient in © 2014 John Wiley & Sons Ltd 2676 Aquaculture Research, 2015, 46, 2676–2684 doi: 10.1111/are.12423