IGF-2 as a predictor of PTD. When IGF-2 was stable over the 2 time frames, there was significantly more likely to be a full term delivery (p ¼ 0.02). CONCLUSION: Circulating biomarker levels as early as the day of the pregnancy test (CD#28) are highly predictive of PTD of a singleton IVF preg- nancy. Supported by: Institutional. O-175 Tuesday, October 21, 2014 05:15 PM SERUM 17-HYDROXYPROGESTERONE (17-OHP) LEVEL ON DAY 7 OF EMBRYO TRANSFER IS A STRONG INDICATOR OF IM- PLANTATION AND A RELIABLE MARKER TO DISTINGUISH BE- TWEEN SINGLETON AND TWIN PREGNANCY IN IVF CYCLES. B. N. Chimote, N. M. Nath, N. N. Chimote, N. M. Chimote. Biochemistry, Embryology, Reproductive Endocrinology, Vaunshdhara Clinic and Assisted Conception Centre, Nagpur, Maharashtra, India. OBJECTIVE: To evaluate 17-hydroxyprogesterone (17-OHP) levels in post embryo transfer serum samples as a determinant of endometrial recep- tivity and conception in IVF cycles. DESIGN: Prospective clinical study without randomization. MATERIALS AND METHODS: Standard ovarian stimulation with antagonist protocol was followed in 336 fresh IVF cycles involving day3/ day5 embryo transfer (ET). Serum levels of 17-OHP, estradiol (E2) and pro- gesterone were measured by radioimmunoassay on days7 and 14 post ET. Luteal phase was Supported with micronized Progesterone injection.Clinical Pregnancy Rate (CPR) was the main outcome measure. Statistical analysis was done using Graph-pad prism V software. RESULTS: 17-OHP levels on day7 (7.260.43 vs. 2.63  0.11 ng/ml, P<0.0001) and day14 (10.310.72 vs. 2.410.22 ng/ml, P<0.0001) of ET were significantly higher in pregnant (n¼98) vs. non-pregnant (n¼238) cy- cles (CPR: 29.17%). The level not only showed a significant rise from day7 to day 14 (7.260.43 to 10.310.72 ng/ml, p¼0.0002) within the preg- nant group but day7 levels were also significantly elevated in cases of twin (n¼25) vs. singleton (n¼73) pregnancies (11.150.86 vs. 5.910.39 ng/ ml, p<0.0001). Clinical Pregnancy rate below and above the median value 2.8 ng/ml was 6.87% (11/160, all singletons) and 49.43% (87/176, 62 single- tons, 25 twins) respectively. Lower cutoff (25th percentile) value of >1.7 ng/ ml increased likelihood of pregnancy (ROCAUC: 96.43%, Sensi- tivity:92.56%, Specificity:75.89%) whereas an upper cutoff (75th percentile) of >4.9 ng/ml enhanced the chances of twin pregnancy by 26.96%. 17-OHP levels did not differ significantly whether embryos were transferred on day3 or day5. Although serum E2 level on Day7 and day14 of ET was significantly higher in pregnant vs. non-pregnant group; its level on day7 of ET did not differ significantly between singleton vs. twin pregnancies. Serum Progester- one levels on d7 and day14 of ET did not differ significantly between non- pregnant and pregnant (singleton as well as twins) groups. CONCLUSION: Serum level of 17-hydroxyprogesterone (17-OHP) on day7 of embryo transfer is a robust indicator of enhanced endometrial response favorable for pregnancy. It is also a distinctive marker to clearly distinguish between a singleton and twin pregnancy early on in an IVF cycle even before the ultrasonograhic visualization of an embryo sac is possible. O-176 Tuesday, October 21, 2014 05:30 PM TO WHAT EXTENT CAN OOCYTES REPAIR SPERM DNA FRAGMENTATION? T. Cozzubbo, Q. V. Neri, Z. Rosenwaks, G. D. Palermo. Reproductive Medicine, Weill Cornell Medical College, New York, NY. OBJECTIVE: To identify the ability/extent of oocytes to cope and eventu- ally repair sperm DNA fragmentation to grant competent embryo develop- ment. DESIGN: In ICSI couples we investigated the role of rising male gamete DFI in relation to embryo development taking into consideration oocyte age as an indicator of DNA repair capability. MATERIALS AND METHODS: We identified couples undergoing infer- tility treatment with recurrent ART failure (n¼115) that had sperm chromatin analysis by TUNEL on their ejaculates. TUNEL was performed by adjusting sperm concentration to 5x10 6 /mL, fixing with 4% PFA, set in permeabilizing solution, and incubated at 37 oC with TUNEL reaction mixture. A minimum of 500 spermatozoa were scored and each patient were deemed normal/ abnormal based on a 15%DFI threshold. Patients were ranked according to increasing level of DFI then categorized according to maternal age. Each subdivision of DFI was evaluated in relation to clinical pregnancy outcome (presence of a fetal heartbeat). RESULTS: This group of men (mean age 39.77yrs) had overall concen- tration of 43.434million/ml, motility of 42.217%, normal morphology of 2.62%, and an average DFI of 14.010% (range 0.8-85.2%). For a specific oocyte age group, we assessed the ability to generate clinical pregnancies once injected with spermatozoa with worsening sperm DFI. There was no clear impairment in pregnancy outcome in relation to the increasing DNA fragmentation. In order to assess an age-related ability to buffer increasing DNA fragmentation, we considered only younger couples. In fact, the % 35 year old women showed no difference in the ability of their embryo to implant, independently of the rising sperm fragmentation rate. Indeed men with a DFI of <10% yielded a pregnancy rate of 26.6% and those with up to 40%DFI, the clinical pregnancy rate was still at 33.3%. When we looked at couples that putatively have an impaired oocyte DNA repair mechanism with a maternal age of >35yrs, the pregnancy rate dropped to 10.0% when the DFI was just up to 20%. CONCLUSION: We currently measure sperm DFI as a factor capable of influencing embryo quality and implantation potential. However, we seem to overlook the DNAse and polymerase activity that the oocyte orchestrate on the male genome during sperm nuclear decompaction. Younger oocytes retained DNA repair ability that can withstand sperm chromatin damage of up to 40% while still granting pregnancies. From this observation we conclude that older oocytes seem to be more sensitive to sperm chromatin damage beginning just above the DFI threshold. Supported by: Reproductive Medicine, Weill Cornell Medical College. O-177 Tuesday, October 21, 2014 05:45 PM ARE EUPLOIDY RATES DIFFERENT IN BLASTOCYSTS FROM DONOR OOCYTES AND THOSE FROM YOUNG INFERTILE PATIENTS? M. Alikani, a D. McCulloh, b J. Barritt, c J. Wilcox, d A. Penzias, e M. Kettel, f S. Munne. g a North Shore University Hospital, Man- hasset, NY; b New York Univ. Fertility Center, New York, NY; c ART Reprod. Center & S. California Reproductive Center, Beverly Hills, CA; d Huntington Reproductive Center, Pasadena, CA; e Boston IVF, Waltham, MA; f Reproge- netics, Livingston, NJ. OBJECTIVE: To compare euploidy rates in blastocysts from donor oo- cytes and those from young infertile patients. DESIGN: Egg donation cycles and cycles from infertile patients <35 years were included in the study. Euploidy rates were compared and stratified according to blastocyst cohort size. MATERIALS AND METHODS: Of 1544 PGS cycles of blastocyst biopsy included, 557 were oocyte donation cycles and 987 were cycles involving infertile patients <35 years. Indications for PGS included male factor, RIF, RPL, or patient’s request. For egg donation cycles, PGS was performed based on patient request or the presence of severe male factor. Biopsied samples were sent from 86 centers to the same reference laboratory and analyzed by array CGH. The data were examined using multiple logistic regression analysis considering the average incidence of euploidy per cycle, maternal age and cohort size. RESULTS: Average number of blastocysts biopsied was higher in egg do- nors than infertile patients (8.4 vs. 6.1; p<0.001). Results are shown in the below table. Overall euploidy rate was significantly higher in egg donors than in young infertile patients (66% vs. 61%; p<0.001). The incidence of euploidy was independent of maternal age within egg donors (avg. age 25.8yrs) and young infertile patients (avg age 31.4yrs) and numbers of bio- psied blastocysts in both young infertile patients and egg donors. Euploidy rates in egg donors and young patients # blastocysts % euploid in Egg donors % euploid in Young Infertile 1-3 58% 61% 4-6 62% 60% 7-10 65% 62% 11-15 68% 63% >15 66% 61% TOTAL 66% 61% CONCLUSION: Euploidy rates are higher in blastocysts from donor oo- cytes than those from young infertile patients. This is both reassuring and consistent with expectations. Although age is a confounder with respect to ploidy, in this young population, it was not found to have an effect. Blastocyst FERTILITY & STERILITY Ò e61