_____________________________________________________________________________________________________ *Corresponding author: E-mail: saroat.ru@gmail.com; Journal of Advances in Microbiology 19(2): 1-6, 2019; Article no.JAMB.52907 ISSN: 2456-7116 Prevalence and Antibiotic Susceptibility of Salmonella from Chicken Eggs in Naogaon District of Bangladesh Md. Saroat Hossain 1* , K. M. Mozaffor Hossain 1 , Md. Mahbubul Alam Sarker 1 and Sm. Ahasanul Hamid 1 1 Department of Veterinary and Animal Sciences, Faculty of Agriculture, University of Rajshahi, Rajshahi-6205, Bangladesh. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JAMB/2019/v19i230187 Editor(s): (1). Dr. Akpaka, E. Patrick, Unit of Pathology and Microbiology, Faculty of Medical Sciences, The University of the West Indies St. Augustine, Trinidad & Tobago. Reviewers: (1). El Baaboua Aicha, Abdelmalek Essaadi University, Morocco. (2). Teresita Sainz Espuñes, UAM-Xochimilco, Mexico. (3). Asitkumar Chakraborty, Vidyasagar University, India. Complete Peer review History: http://www.sdiarticle4.com/review-history/52907 Received 19 September 2019 Accepted 22 November 2019 Published 28 November 2019 ABSTRACT Salmonella is one of the most common zoonotic bacteria that cause foodborne illness in humans. An investigation was conducted to determine the prevalence and antibiotic susceptibility patterns of Salmonella isolates from chicken eggs in the Naogaon district, Bangladesh. Salmonella was isolated from cultures on different selective-differential media and further identified by biochemical tests. Antibiogram study was done by the disk diffusion method. The overall prevalence of Salmonella was recorded as 7.78%, whereas 5.56% was on eggshell surfaces and 2.22% was in egg contents. The Salmonella prevalence was 8.33%, 13.33% and 1.67% in chicken eggs from layer farms, whole sellers and retailers, respectively. Salmonella isolates were found 50.0% to 85.71% sensitive to chloramphenicol, gentamycin, ciprofloxacin, and ceftriaxone. Resistance against gentamycin, chloramphenicol, and ampicillin was found significant ranging from 21.43% to 71.42%. The highest resistance was found in amoxicillin (92.86%). The present study proposes that chicken eggs are a potential reservoir of multidrug-resistant Salmonella. Antibiotic-resistant Salmonella will pose a problem to treat Salmonella infection in humans. Thus, the aim of this study is to assess the risk of Salmonella resistance in chicken eggs. Original Research Article