Article Topology, Glycosylation and Conformational Changes in the Membrane Domain of the Vacuolar H + -ATPase a Subunit † Norbert Kartner 1 , Yeqi Yao 1 , Ajay Bhargava 1 and Morris F. Manolson 1,2* 1 Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario M5G 1G6, Canada. 2 Department of Biochemistry, Faculty of Medicine, University of Toronto, Toronto, Ontario M5S 1A8, Canada. RUNNING HEAD: V-ATPase a subunit topology KEY WORDS: • ATPases • Bioenergetics • Proton pumps • Proton transport • Protein structure The authors declare no conflicts of interest. Contract grant sponsor: Canadian Institutes of Health Research; Contract grant numbers: MOP- 111195 and SOW-90384. * Corresponding author: Faculty of Dentistry, University of Toronto, 124 Edward St., Toronto, Ontario M5G 1G6 Canada, Tel. 416-979-4900, Fax. 416-979-4936; E-Mail: m.manolson@dentistry.utoronto.ca † This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: [10.1002/jcb.24489] Received 24 June 2012; Revised 26 November 2012; Accepted 21 December 2012 Journal of Cellular Biochemistry © 2013 Wiley Periodicals, Inc. DOI 10.1002/jcb.24489