BOHR Journal of Biocomputing and Nano Technology 2023, Vol. 1, No. 2, pp. 30–33 DOI: 10.54646/bjbnt.2023.06 www.bohrpub.com METHODS Implanting decellularized plant tissue containing CAR-T cells at debulked tumor sites K. Hariharan 1 , S. Bharathraj 2 , Immanuel Riches 3 and Kirubanandan Shanmugam 3 * 1 Veltech University, Chennai, India 2 Department of Biotechnology, Sree Sastha Institute of Engineering and Technology, Chennai, India 3 International Conference on Modern Advancement in Diagnosis and Treatment of Clinical Cancer, Sree Sastha Institute of Engineering and Technology, Chennai, India *Correspondence: Kirubanandan Shanmugam, kirubabio@ssiet.in Received: 11 May 2023; Accepted: 24 May 2023; Published: 31 May 2023 Surgical treatment of cancer includes the removal of the entire tumor. Sometimes, surgery removes some, but not all, of cancer tumors regarding the safety of the organ. This is called debulking. The debulked site contains some cancer cells. CAR-T cell therapy is a cancer immunotherapy that uses T cells of patients to fight against cancer cells. T cells collected from patients’ blood are genetically modified using chimeric antigen receptor (CAR) gene. The modified T cells are grown in a culture medium. Decellularization is the process of sterilizing a preexisting natural organ to the extent that only the extracellular matrix scaffold base remains. The CAR-T cells injected decellularized plant tissues are used as an effective treatment against cancer cells present at debulked tumor sites. Keywords: cancer, tumor, surgery, debulking, CAR-T, T cells, chimeric antigen receptor (CAR), extra cellular matrix scaffold Introduction Radiation, chemotherapy, and surgery are the most commonly used cancer treatments. Targeted therapy, immunological therapy, laser, hormonal therapy, and other methods are also available. Many forms of cancer are commonly treated with surgery. Surgical treatment is done by two methods, namely, the entire removal of tumor or partial removal of tumor. The partial removal of tumor will not ensure the complete removal of the cancer cells. Some cancer cells may present at the tumor sites. This is called debulking. The term “chemotherapy” (“chemo”) is frequently used to describe treatments for cancer. However, not all cancer medications function in the same way. The medications used in conventional or traditional chemotherapy are cytotoxic, which means they can destroy tumor cells. Knowing how traditional or standard chemotherapy functions and what to anticipate may frequently assist you in preparing for treatment and, if necessary, in making sensible healthcare decisions. Immunotherapy is a type of cancer treatment that helps your immune system fight cancer. Your body’s immune system works to keep you healthy and free from infections and diseases. Organs, lymphatic system components, and white blood cells are some of its elements. One example of a biological treatment is immunotherapy. Biotherapy is a type of cancer treatment that uses elements taken from living things to fight the disease. Tumor antigens, which immune system antibody proteins can identify and bind, are often found in cancer cells, which make cancer immunotherapy possible. Frequently, proteins or other large molecules serve as tumor antigens (e.g., carbohydrates). Modified immunotherapy antibodies, in contrast to conventional antibodies, which bind to external pathogens, recognize and mark cancer cells for the immune system to suppress or kill. Decellularization Plant decellularization A nearby store provided the spinach and parsley. Leafy Artemisia annua plants that were growing on the ground 30