~ 706 ~ Journal of Entomology and Zoology Studies 2018; 6(1): 706-709 E-ISSN: 2320-7078 P-ISSN: 2349-6800 JEZS 2018; 6(1): 706-709 © 2018 JEZS Received: 06-11-2017 Accepted: 07-12-2017 Dr. Harshit Verma Assistant Professor, Department of Veterinary Microbiology College of Veterinary & Animal Sciences, S.V.P. University of Agriculture & Technology, Meerut, Uttar Pradesh, India Dr. Shriya Rawat Assistant Professor Department of Veterinary Public Health & Epidemiology College of Veterinary & Animal Sciences S.V.P. University of Agriculture & Technology, Meerut, Uttar Pradesh, India Nishant Sharma Veterinary Officer, Uttarakhand Govt, College of Veterinary and Animal Sciences, Sardar, Vallabhbhai Patel University of Agriculture and Technology, Modipurum, Meerut, Uttar Pradesh, India Dr. Vikas Jaiswal Assistant Professor, Department of Veterinary Pathology, College of Veterinary & Animal Sciences, S.V.P. University of Agriculture & Technology, Meerut, Uttar Pradesh, India Dr. Rajeev Singh Associate Professor & Head, Department of Veterinary Microbiology, College of Veterinary & Animal Sciences S.V.P. University of Agriculture & Technology, Meerut, Uttar Pradesh, India Correspondence Dr. Harshit Verma Assistant Professor, Department of Veterinary Microbiology, College of Veterinary & Animal Sciences, S.V.P. University of Agriculture & Technology, Meerut, Uttar Pradesh, India Prevalence, bacterial etiology and antibiotic susceptibility pattern of bovine mastitis in Meerut Harshit Verma, Shriya Rawat, Nishant Sharma, Vikas Jaiswal and Rajeev Singh Abstract Increasing antimicrobial resistance has become a big challenge worldwide. Mastitis is the most common disease for antibiotic use in dairy herds and thus, antimicrobial resistance of mastitis pathogens has received recent attention. The objective of the current study was to assess the status of bovine mastitis in and around Meerut region, bacterial pathogen involved and to determine the susceptibility of different antibiotics. A total of 85 milk samples were presented to Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Meerut from April, 2016 to June, 2017 with a history of swelling in udder, loss of milk, flakes formation in milk, RBC in milk and watery milk. The milk samples were cultured on brain heart infusion agar, MacConkey’s agar, eosin methylene blue agar and sabrourad dextrose agar. The isolated organisms through microbiological procedures were subjected to antimicrobial susceptibility test by disc diffusion method to a twelve number of antibiotics. The major prevalent pathogens isolated were Staphylococcus Spp. (42.55%), E. coli (21.28%), Streptococcus spp. (6.38%), Proteus spp. (8.51%), Candida spp. (2.88%) and mixed infection (18.26%). The present results revealed that gentamicin (65.96%) was the most effective antibiotic followed by enrofloxacin (63.83%), cefotaxime+clavulanic acid (52.13%), amoxicillin+sulbactum (42.55%), ciprofloxacin (41.49%), colistin (41.49%), chloramphenicol (39.36%) and ampicillin+sulbactum (38.29%). Least effective drugs were oxytetracycline (22.34%), streptomycin (25.53%) whereas maximum resistance drug were found amoxyclave (8.51%), and ampicillin/cloxacillin (8.51%). Keywords: Mastitis, antibiotics, disk diffusion Introduction Acquired antimicrobial resistance in bacteria has been a growing concern worldwide [1] . The era of overuse and misuse has lead to the evolution of resistant forms of previously harmless bacteria. Mastitis is one of the most costly and important disease of dairy industry and antimicrobials are important part of therapy [2] . Mastitis is a multi-etiological and complex disease, which is defined as inflammation of parenchyma of mammary glands and represents one of the most difficult veterinary diseases to control [3] . Mastitis adversely affects animal health, quality of milk and the economics of milk production, affecting every country, including developed ones and causes huge financial losses [4] . One important reason for treatment failure is assumed to indiscriminate use of antibacterials without testing in-vitro sensitivity of causal organisms [5] . Bovine mastitis is classified as: contagious mastitis, generally caused by contagious bacteria presiding on the skin of the teat and inside the udder, transmitted from one cow to another during milking (e.g., Staphylococcus aureus or Streptococcus agalactiae) and environmental mastitis, caused by environmental pathogens normally found in shed surroundings such as bedding, manure, soil, and feed (e.g., Escherichia coli, Streptococcus uberis, Klebsiella sp.) [6] . It has been estimated that the mastitis alone can cause approximately 70% of all avoidable losses incurred during milk production. In India, annual economic loss to dairy industry due to subclinical mastitis and clinical mastitis is estimated to be Rs. 7165.5 crores [7] . The occurrence of disease is an outcome of interplay between three major factors: infectious agents, host resistance, and environmental factors [8] . Multi-drug resistant bacteria are a persistent problem in modern health care, food safety and animal health. There is a need for new antimicrobials to replace over used conventional antibiotics [9] . Therefore, a bacteriological diagnosis, prevalence study in the herd and proper selection of antibiotic based on antibiotic sensitivity are critical for rational and effective control of mastitis.