Copper Nanoparticles Impregnation in anti-Coronavirus Covid’19 Disposable Protective Masks G. Margraf 1 , B. L. Fernandes 1,2 , P. Nohama 1,2 1 PPGTS-PUCPR, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil 2 Department of Biomedical Engineering, Pontifícia Universidade Católica do Paraná, PR, Brazil E-mail: gabriel_margraf_8@hotmail.com Abstract –– After two pandemics in less than a decade, it is clear that the fight against microbes is failing, and one of the reasons is the inefficacy of disposable protective masks. This project intends to evaluate the possibility of the impregnation of copper particles as an active element to eliminate viruses and bacteria, which in contact with cotton conventional masks have a survival rate of up to 72 h. In contact with copper particles, those microorganisms survive, approximately, for 1 h. Therefore, the main objective of this work was to evaluate a production process of impregnation of copper particles in nonwoven fabric (TNT). To do so, we isolated copper particles from copper sulfate solution with constant heating, simultaneously impregnating pieces of TNT cut from commercial disposable protective masks. We performed SEM and EDS analysis to evaluate the impregnation and particle sizes. Visually, the pieces were partially impregnated which was confirmed by the SEM images where one can see only some fibers covered by the particles. Also, it is possible to notice that the size of the particles was at microscale with some agglomeration. The EDS confirmed 1.1% by mass of copper particles in the sample. The procedure was easy to follow and at a low-cost being, therefore, possible to be implemented after some parameter adjustments. Keywords –– Pandemics, copper, masks I. INTRODUCTION The moment in which humanity lives needs the convergence of the efforts of the academic community aimed at the innovation or improvement of techniques that help in the fight against the COVID-19 pandemic, as well as others to come [1]. In this scenario, an indispensable accessory is the disposable protective masks, used by health professionals and the population in general. However, in addition to not promoting the total retention of particles [2], it does not provide the elimination of microorganisms. Therefore, efforts have been done to develop a more effective barrier in masks and equipment, guaranteeing better protection and, as a consequence, a lower spread of diseases caused by pathogenic microorganisms [3,1]. Based on reports of scientific studies, as well as on historical and contemporary empiricism of the effectiveness of the use of copper nanoparticles as agents to combat viruses and bacteria [4,1,5,3], we performed an experiment aimed to develop new copper-based particles impregnating method in TNT pieces of disposable protective masks. II. METHODOLOGY We prepared one 2 g/l aqueous solution of copper sulfate (CuSO4) and another containing 10 g/l ascorbic acid solute (C6H8O6). The two solutions were mixed in the following proportion: 500 ml of the copper sulfate solution and 100 ml of the ascorbic acid solution. Some square pieces of TNT surgical masks (2 cm side) were added to the mixture of solutions inside a clean and ethanol 70% sterilized 1 L round-bottom volumetric flask with three outlets (Fig 1.). The system was heated to 80 °C, in a glycerin bath for 2 h. Fig. 1. The mixture in the volumetric flask (arrow) ϮϬϮϮ '>K> D/> E'/EZ/E' W,z^/^ y,E'^WE DZ/E ,>d, Z y,E'^ ;'DWW,Ϳ /^E 978-1-6654-8435-0 / ĂƚĂůŽŐ EƵŵďĞƌ &WϮϮϭϴ'ͲZd WED /dz WED DZ, Ϯϭ Ͳ Ϯϲ ϮϬϮϮ © IEEE 2022. This article is free to access and download, along with rights for full text and data mining, re-use and analysis. 2022 Global Medical Engineering Physics Exchanges/ Pan American Health Care Exchanges (GMEPE/PAHCE) | 978-1-6654-8435-0/22/$31.00 ©2022 IEEE | DOI: 10.1109/GMEPE/PAHCE55115.2022.9757790