OA34 211. EVALUATION OF AYURVEDIC TREATMENT ALONG WITH CONVENTIONAL MODERN ANTI-HYPERGLYCEMIC DRUG IN THE MANAGEMENT OF DIABETIC SENSORY PERIPHERAL POLYNEUROPATHY e AN INVESTIGATOR BLINDED RANDOMIZED CONTROLLED TRIAL Manish Patel, Daniel Scheidbach, Bhumika Patel, Mansi Patel, Kalapi Patel, S.N. Gupta. Objective: Therapy of diabetic sensory polyneuropathy has not been satisfactorily addressed in modern medicine till today. A traditional Ay- urvedic treatment has been observed to show satisfactory benefit but has not been compared versus placebo treatment. Hence, a randomized, investigator blinded, placebo-controlled trial was conducted to evaluate the efficacy of Ayurvedic treatment. Methods: Patients of diabetic sensory peripheral polyneuropathy were randomized to Ayurvedic treatment (decoction made from 10 gm of sida cordifolia root and 3 gm powder of phyllanthus nirruri twice in a day) or placebo for 3 weeks period. All the patients were instructed to take the conventional anti-hypertensive drugs as per their routine scheduled. A neuropathy analyzer device was used for detection of vibration and thermal sensation threshold while symptoms were assessed using Neu- ropathy total symptom score 6 (NTSS-6). Results: 30 patients of each group completed the treatment course. Pa- tients receiving Ayurvedic treatment were significantly got symptomatic response at week 3 according to NTSS-6 (P < 0.001 in all the symptoms for Ayurvedic treatment vs. placebo). Vibration, hot and cold sensation of right foot were improved by 19.4%, 2.8% and 11.2% and of left foot by 20%, 4.9% and 17.6% respectively as they came in lower range of moderate affection from severe in Ayurvedic treatment group while they were remain un- changed in placebo group. Conclusion: Patients of diabetic sensory peripheral polyneuropathy treated with Ayurvedic medicine were more likely to achieve clinical response than those receiving placebo. Long term use of Ayurvedic treat- ment may be more beneficial in these cases. OA35 215. EVALUATION OF HEPATOPROTECTIVE AND NEPHROPROTECTIVE POTENTIAL OF BILVADI GUTIKA IN CYPERMETHRIN INDUCED TOXICITY IN WISTAR ALBINO RATS A.K. Neeraj, U.N. Prasad, Swapna Swayamprabha. Introduction: Bilwadi Gutika is explained as one of the formulations in the context of Ayurvedic snake bite management. It is clinically used in various conditions such as Bhujanga Visha (snake bite), Luta Visha (spider poison), Unduru Visha (rat bite), Vrischika Visha (scorpion sting), Visuchika (Cholera), Ajirna (indigestion), Gara Visha (artificial poison), and Jwara (fever). This Gutika have shown hepatoprotective and nephroprotective activity, and a study was conducted to evaluate the hepatoprotective and nephroprotective effect of Bilwadi Gutika in cypermethrin induced hepa- totoxicity in Wistar rats. Aim: To evaluate the hepatoprotective and nephroprotective potential of Bilwadi Gutika in cypermethrin induced toxicity in wistar albino rats. Materials and methods: The sub acute toxicity was induced and effect of Bilwadi Gutika was evaluated according to OECD guidelines 407, in wistar albino rats. Results: Significant reversal of the toxicant induced changes in the food intake and conversion related parameters were observed at both the dose level. In antioxidant study, significant effect of test drug was seen on lipid peroxidation and antioxidant parameters. At lower dose level degenerative changes in kidney and liver were found to be not reversed but higher dose produced moderate reversal. Conclusion: The results of this study revealed that even though significant reversal of the toxicant induced changes were observed in antioxidant parameters, the food intake and conversion related parameters. The beneficial effects of Bilwadi Gutika can be used for therapeutic purpose for the treatment of toxicity due to Cypermethrin along with another cyto- protectant. OA36 216. AN EXPERIMENTAL STUDY TO ASSESS THE ANTI-OSTEOPOROTIC EFFECT OF PANCHATIKTA GHRITA (PG) IN AN OVARIECTOMIZED RAT MODEL OF OSTEOPOROSIS Renuka Munshi, Samidha Joshi, Dipti Kumbhar. Purpose: The Study was conducted to evaluate the anti-osteoporotic effect of Panchatikta ghrita (PG) in an ovariectomized rat model of Osteoporosis Method: Institutional Animal Ethics Committee approval was obtained prior to study initiation. 96 female Sprague Dawley rats (260 gms weight) were divided into 8 study groups viz. Sham control group (NC), Disease control (DC), Vehicle control (VC), 3 test drug [Panchatikta Ghrita (PG)] groups (TC1, TC2 & TC3 e 0.9, 1.8 and 2.7 gm/kg body weight respectively], Standard control 1 (SC1) received 17a-ethinyl estradiol 1 mg/kg/day and (SC2) received Alendronate (7 mg/kg/week). All the rats received the drugs daily for 4 months. Bone specific biomarkers viz. Osteocalcin and Trap-5b were estimated at baseline and end of study. Animals were sacrificed on day 121 and their femurs and tibiae harvested for Histomorphometric analysis and bone mineral density (BMD) studies. Research: Increase in Osteocalcin & Trap-5b levels was observed in DC group as compared to NC. All 3 doses of Panchtikta ghrita (PG) decreased Osteocalcin & Trap-5b levels with maximum effect seen with the highest dose of PG which was similar to the standard drugs All the 3 doses of PG increased BMD however the maximal effect was seen at 1.8 gm/kg/wt dose. A significant decrease in a number of osteoclasts, whereas an in- crease in bone hardness and elasticity was observed as compared to diseased group demonstrating anti-osteoporotic effect of PG. Conclusion: PG increased bone specific markers, BMD and decreased osteoclast formation, indicating its anti-osteoporotic potential. OA37 218. EVALUATION OF PROTECTIVE ROLE OF BILWADI GUTIKA IN LEAD INDUCED TOXICOPATHOLOGICAL CONDITION IN WISTAR ALBINO RATS P.U. Shubha, V. Sudheendra Honwad, R. Shrinidhi. Introduction: Lead is one of the leading cause for toxicity since ancient roman time. Lead toxicity can be compared with the concept of Garavisha. Bilwadi Gutika is a formulation mentioned in Ashtanga Hrudaya under the context of sarpa visha chikita which is widely used by the keraliya visha vaidya in Vishaja conditions like Vruschika Visha, Undura Visha, Gara visha etc. Materials and methods: Literature on lead, concept of Gara Visha, & Bilwadi Gutika were collected from classical books, modern books & websites. Bilwadi Gutika (Test Drug) was procured from Arya Vaidya Sala kottakal, Kannur branch and test drug was administered orally for 28 days. The repeated dose toxicity was carried out at 2 different dose levels e TED & TED x 2. Parameters like biochemical, antioxidant activity & histopathological tests are compared with control group for toxicity reports. Results: All the parameters like biochemical, anti-oxidant activity & his- topathological findings showed moderate toxicity by lead acetate. In Bil- wadi Gutika TED group results were not much evident, but in Bilwadi Gutika TED 2 group significant reversal of toxicity was observed. Conclusion: Analysis of the data obtained during the study indicates the lead acetate at the dose level used induced moderate severity toxic effects. Ma- jority of them were reversed by the Bilwadi Gutika providing evidence for its utility as a universal anti-dote. It can be gainfully clubbed with other mode of therapies or other formulations for effective treatment of lead toxicity. OA38 225. ASSESSMENT OF TRAILOKYASUNDAR RASA e A HERBO MINERAL FORMULATION FOR ITS EFFICACY AGAINST CHRONIC MYELOID LEUKEMIA IN AN IN VIVO MODEL Paras Golecha, Siddhisha Kurve, Sheela Pargunde. Purpose: Trailokyasundar rasa is one of the rasakalpa which is explained in rasaratnasamucchaya, and is suggestive of its efficacy in Pandu roga. Abstracts / Journal of Ayurveda and Integrative Medicine 9 (2018) S1eS30 S9