280 Copyright © All rights are reserved by Abdul M Gbaj. Drug Designing & Intellectual Properties International Journal Research Article ISSN: 2637-4706 An in vivo study for the effect of Citrus reticulata (Rutaceae) fruit peels extracts on the onset of toxicity of Cerastes cerastes venom in Albino mice Mohamed A Gbaj 1 , Inass A Sadawe 2 , Nisreen H Meiqal 2 , Salah M Bensaber 2 , Massaud Salem Maamar 3 , Anton Hermann 4 and Abdul M Gbaj 2 * 1 Department of Chemical Engineering, University of Tripoli, Libya 2 Department of Medicinal Chemistry, University of Tripoli, Libya 3 Zoology Department, Tripoli University, Libya 4 Department of Biosciences, University of Salzburg, Austria *Corresponding author: Abdul M Gbaj, Department of Medicinal Chemistry, University of Tripoli, Libya Received: April 03, 2019 Published: April 25, 2019 DOI: 10.32474/DDIPIJ.2019.03.000153 Abstract Venom of Cerastes cerastes has been extracted and its toxicity was investigated in the presence of aqueous and methanolic extracts of Citrus reticulata (Rutaceae) fruit peels. The decline in the mean survival time of the male albino swiss mice were used to deduce the venom property in the presence and absence of aqueous and methanolic extracts of Citrus reticulata (Rutaceae) fruit peels. The aqueous and methanolic extracts of Citrus reticulata (Rutaceae) fruit Peels significantly decrease the mean survival time compared to the venom alone. From these results it was evident that the toxicity of Cerastes cerastes venomis increased significantly in the presence of Citrus reticulata in a dose dependent manner Keywords: Citrus reticulata; Cerastes cerastes; Venom; Toxicity Introduction Snakebites are severe socio-medical difficulty that lead to morbid and fatal affect on victims in Libya and other North African countries [1,2]. Immediate antivenom treatment is crucial and vital to avoid morbidity and mortality [3]. The oxidative trauma condition, which result from snake bite envenomation is another measurement of kidney destruction and severe renal failure [4], connected with the antioxidant defense system, that might be subject for treatment by antioxidant therapy [5]. ROS (Reactive oxygen species) are engaged in many inflammatory reactions, thus influencing the physiology of the cells and participate a significant function in the pathological conditions [6]. As have been free radical, ROS are involved in harming cellular components, and they play an important function in venom induced toxicity, as reported among envenomed mice [7]. Ascorbic acid is an antioxidant that has been reported to have useful effects on a number of cancer types [8,9] and could be concerned in alleviation of Reactive oxygen species cellular damage, produced during exposure to toxins, metabolism and carcinogens [10]. In addition to augmentation of protease inhibitor effects concerned in preventing organ efficient injure [11,12]. Citrus reticulata (Rutaceae) is commonly known as narangi or santra (orange). It is a small spiny tree with thick top of slim branches, extensively grown in Egypt, Tunisia and Libya [13]. Mandarin is a collection name for this class of orange with thin, loose peel. The name ‘tangerine might be applied as an interchange name to the entire group, but in trade, it is usually limited to the types with red-orange skin. The fruit has aphrodisiac, laxative, tonic and astringent properties [14,15]. It is also used to alleviate vomiting [16,17]. The fruit peel controls the skin moisture, rough and softens hard skin and possess a cleaning effect on oily skin [18]. Chemical composition of the volatile oil of the fruit peels of this species has been reported [19-23]. The effects of the volatile oil of C. reticulata has been studied against Saccharomyces cerevisiae [24], pathogenic fungi, Paenibacillus larvae, Schistosoma mansoni, Aspergillus flavus, and other microorganisms [25-30]. Very recently, the volatile oil of C. reticulata also demonstrates anticancer activity [31-33]. The main aim of the current study is to investigate the effects of Citrus