-1- ABSTRACT Neobalanocarpus heimii (Cengal) is from the family Dipterocarpaceae. It is a long- lasting wood that is also one of the most robust timbers in the world. This species is native to Peninsular Malaysia and southern Thailand. In this study, the Cengal heartwood was studied concerning the amount of water extractive content with antifungal properties from the Neobalanocarpus heimii. The dilution method was used to test the antifungal properties. Wood meals samples were subjected to the se- quential extractive beginning with hexane followed by dichloromethane, methanol and water. The extracts were collected and underwent evaporation by using rotary evaporator to obtain pure crude extract. The antifungal activities were determined using agar dilution method. Two selected fungi Trametes versicolor (T. versicolor) and Coniophora puteana (C. puteana) were used. The antifungal index (%) which compares the diameter of the growth zone for the experimental plate and control plate was calculated. The total percentage of yield from Neobalanocarpus heimii was 0.28%. The highest antifungal index obtained for Trametes versicolor (T. versicolor) was 81.22%, while C. puteana was 43.24%. The crude extracts from Neobalanocarpus heimii were effective in inhibiting the growth of Trametes versicolor and Coniophora puteana. Copyright: © 2022, J. Tropical Biodiversity Biotechnology (CC BY-SA 4.0) Research Article Antifungal Activities of Neobalanocarpus heimii (Cengal) Heartwood Extracts on Trametes versicolor and Coniophora puteana Nur Afiqah Manan 1 , Ismail Jusoh 2 , Furzani Pa’ee 1 * 1) Department of Technology & Natural Resources, Faculty of Applied Sciences & Technology, Universiti Tun Hussein Onn Malaysia (UTHM)Pagoh Campus , Pagoh Educational Hub, KM 1, Jalan Panchor, 84600 Panchor, Johor. 2) Universiti Malaysia Sarawak, Jalan Datuk Mohammad Musa, 94300 Kota Samarahan, Sarawak * Corresponding author, email: furzani@uthm.edu.my Journal of Tropical Biodiversity and Biotechnology Volume 07, Issue 03 (2022): jtbb69942 DOI: 10.22146/jtbb.69942 INTRODUCTION The traditional toxic wood preservatives that create environmental hazards can be replaced by secondary metabolites of timber with antifungal proper- ties and can be used as natural biodegradable fungicides. Antifungal refers to a chemical compound produced biosynthetically or synthetically that could destroy or usefully suppress the metabolism of various harmful microscopic organisms (Kawamura et al. 2010). An abundance of naturally durable wood species demonstrates im- proved efficiency and durability in outdoor exposure without preservatives. When assessing and forecasting the efficiency of a naturally durable wood species, wood extractives are widely considered to be a contributing factor (Kirker et al. 2013). Wood extractives are the non-structural components of wood. It is often produced by the standing tree as defensive compounds to Keywords: Antifungal activities dilution heartwood Neobalanocarpus heimii Trametes versicolor Coniophora puteana Submitted: 24 October 2021 Accepted: 19 July 2022 Published: 12 October 2022 Editor: Miftahul Ilmi