ISSN: 0975-8585 November - December 2015 RJPBCS 6(6) Page No. 1364 Research Journal of Pharmaceutical, Biological and Chemical Sciences Isolation and Characterization of Cr 3+ resistant Klebsiella sp Shine2. C Shiny Matilda, and C Shanthi*. School of Bioscience and Technology, VIT University, Vellore-632014, Tamil Nadu, India. ABSTRACT A bacterium isolated from the tannery effluents with an ability to resist Cr 3+ upto a concentration of 1000ppm was identified as Klebsiella sp Shine2 by 16S rRNA gene sequencing. Growth curve study indicated increase in lag phase duration with increasing concentration of the metal. The isolate exhibited resistance to antibiotics namely, gentamicin, amoxicillin, ampicillin, erythromycin, carbenicillin, tetracycline and chloramphenicol and also possessed plasmid of size 10kb. Screening for proteolytic activity of the organism using skim milk agar plates showed clear zones around colonies. Owing to the metal resistant property of the isolate, protease from the organism could be used for environmental and industrial applications. Keywords: Klebsiella, trivalent chromium, Metal Resistance, Protease *Corresponding author