Rev. Colomb. Cienc. Quím. Farm., Vol. 50(3), 601-632, 2021 www.farmacia.unal.edu.co 601 Scientific research article / http://dx.doi.org/10.15446/rcciquifa.v50n3.93283 Spray dried extract of Calophyllum brasiliense Cambés produces a potent hypoglycemic effect and improves total serum cholesterol and triglycerides in diabetic rats Jesús Rafael Rodríguez Amado 1a , Ariadna Lafourcade Prada 2b , Bianca Rodrigues Acacio 1c , Verónica Bautista Robles 1d , Hady Keita 1 , Tatiane Pereira de Souza 2e 1 Federal University of Mato Grosso do Sul, Campo Grande, Brazil. ²Federal University of Amazonas, Manaus, Brazil. E-mail adresses: a jesus.rafael@ufms.br b ariadnalafu1977@gmail.com c bianca.r.acacio@gmail.com d robles_verosav@hotmail.com e tpsouza@ufam.edu.br Received: February 2, 2021 Corrected: March 15, 2021 Accepted: March 19, 2021 Summary Introduction: e species Calophyllum brasiliense Cambés (Calophyllaceae) is widespread throughout Central and South America. e stem bark infusion is used for lowering blood glucose. Aim: To optimize the spray dry extract of this plant using a D-optimal experimental design. Materials and methods: As factors were used the air-drying speed (3.5-4.5 m 3 /h), the feed flow rate of the suspension (5-11 mL/ min), and the inlet air temperature (90-130 °C). e dried extract was character- ized by measuring the phenolics and flavonoids content, moisture, the water activity, apparent densities, flowability, and compressibility. e antioxidant activity, the inhibitory activity of lipase and alpha-glycosidase, and the antiglycant activity of the spray dried extract (SDE) were evaluated. Subsequently, the hypoglycemic activity was evaluated in rats by monitoring the blood glucose level, triglycerides, and choles- terol. Results: Inlet air temperature and feed flow rate were the factors that most affected the yield and phenolic content. SDE showed a potent antioxidant effect