Prevalence of Acinetobacter baumannii harboring ISAba1/bla OXA-23-like family in a burn center Himen Salimizand a,b,d , Neda Noori d , Zahra Meshkat c,d, *, Kiarash Ghazvini c,d , Saeid Jamehdar Amel d a Student Research Committee, Department of Microbiology & Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran b Department of Microbiology and Virology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran c Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran d Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran b u r n s x x x ( 2 0 1 5 ) x x x – x x x a r t i c l e i n f o Article history: Accepted 10 December 2014 Keywords: Acinetobacter baumannii Burn ISAba1/blaOXA23-like REP-PCR a b s t r a c t Background: Refractory carbapenem resistant Acinetobacter baumannii (CRAB) isolates in- crease mortality and morbidity rates among patients with underlying disorders, especially in patients with burns. The aim of the current study was to understand the resistant determinants of CRAB isolates and their clonal relatedness collected from referral Burn center. Methods: CRAB isolates were initially characterized and then antimicrobial susceptibility testing was assessed by E-test. Resistance determinants were investigated by PCR. Repeti- tive extragenic palindromic elements-PCR (REP-PCR) was used for clonality relatedness among isolates. Results: Thirty CRAB isolates were collected during the study. Colistin was the most effective antibiotic, but, all of the isolates were resistant to carbapenems. intI1 was detected in two isolates and MBLs and gene cassettes were not detected. ISAba1, bla OXA-51 , bla OXA-23 and ISAba1/bla OXA23-like were detected in all, while bla OXA-24-like were present in 73% and bla OXA-58 & OXA-143 were not present in isolates. REP-PCR demonstrated three clusters, with the domi- nant B cluster, which contained 16 subgroups. Conclusion: CRAB harboring ISAba1/bla OXA-23-like family is widely disseminated in the studied Burn ward setting and the emergence of infection control measures should be regarded to limit refractory CRABs. # 2014 Elsevier Ltd and ISBI. All rights reserved. * Corresponding author at: Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, (Ghaem Hospital), Department of Microbiology & Virology, Faculty of Medicine, Shariati Square, Ahmadabad Boulevard. PO Box: 91766-99199 (155), Mashhad, Khorasan Razavi, Iran. Tel.: +98 513 8012453; fax: +98 513 8002287. E-mail address: meshkatz@mums.ac.ir (Z. Meshkat). JBUR-4547; No. of Pages 7 Please cite this article in press as: Salimizand H, et al. Prevalence of Acinetobacter baumannii harboring ISAba1/bla OXA-23-like family in a burn center. Burns (2015), http://dx.doi.org/10.1016/j.burns.2014.12.008 Available online at www.sciencedirect.com ScienceDirect journal homepage: www.elsevier.com/locate/burns http://dx.doi.org/10.1016/j.burns.2014.12.008 0305-4179/# 2014 Elsevier Ltd and ISBI. All rights reserved.