Bioactivity of gel– glass powders in the CaO-SiO 2 system: A comparison with ternary (CaO-P 2 O 5 -SiO 2 ) and quaternary glasses (SiO 2 -CaO-P 2 O 5 -Na 2 O) Priya Saravanapavan, Julian R. Jones, Russell S. Pryce, Larry L. Hench Tissue Engineering Centre, Department of Materials, Imperial College of Science, Technology and Medicine, Prince Consort Road, London SW7 2BP, United Kingdom Received 22 March 2002; revised 18 July 2002; accepted 16 August 2002 Abstract: Bioactive glasses react chemically with body flu- ids in a manner that is compatible with the repair processes of the tissues. This results in the formation of an interfacial bond between the glasses and living tissue. Bioactive glasses also stimulate bone– cell proliferation. This behavior is de- pendent on the chemical composition as well as the surface texture of the glasses. It has been recently reported that gel-derived monolith specimens in the binary SiO 2 CaO are bioactive over a similar molar range of SiO 2 content as the previously studied ternary CaO-P 2 O 5 -SiO 2 system. In this report, the preparation and bioactivity of the binary gel– glass powder with 70 mol % SiO 2 is discussed and its bio- activity is compared with the melt-derived 45S5 (quaterna- ry) Bioglassand sol– gel-derived 58S (ternary) bioactive gel– glass compositions. Dissolution kinetic parameters K 1 and K 2 were also computed based on the silicon release for all glass powders. It was shown that the simple two-com- ponent SiO 2 -CaO gel– glass powder is bioactive with com- parable dissolution rates as the clinically used melt-derived 45S5 Bioglasspowder and extensively studied sol– gel-de- rived 58S gel– glass powder. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 66A: 110 –119, 2003 Key words: porosity; in vitro bioactivity; CaOSiO 2 ; gel– glass powders; Bioglass INTRODUCTION A bioactive material is one that elicits a specific biological response at the interface of the material, which results in the formation of a bond between the tissue and the material. 1 This concept is based upon control of the surface chemistry of the material. A bioactive implant reacts chemically with body fluids in a manner that is compatible with the repair pro- cesses of the tissues. The first bioactive material reported was a four- component glass composed of SiO 2 , CaO, Na 2 O, and P 2 O 5 by Hench et al. in 1971. 2 The composition of Bioglass45S5, which has been in clinical use for the last 15 years, is given in Table I. When implanted, the low silica content and the presence of sodium ions in the glass results in very rapid ion exchange with the protons and hydronium ions of physiological solu- tions. 3 The ion exchange creates an alkaline pH at the implant interface with the body fluids, leading to nu- cleation and crystallization of hydroxyl carbonate apa- tite (HCA) bone mineral at the surface of the glass. The growing bone mineral layer bonds to collagen, pro- duced by the bone cells, and forms a strong interfacial bond between the implant and the living tissues. 1,2 During the last 30 years, extensive research on bio- active glasses and glass– ceramics has been conducted. Many compositions containing SiO 2 , CaO, and P 2 O 5 are found to be biologically active. They include Cer- avital, 4 CeraboneA/W glass ceramics, 5– 8 -tricalcium phosphate, 9 sintered hydroxy apatite, 10 58S bioactive gel– glasses, 11–14 modified 45S5 glass compositions, 15 and bioactive composites. 16 Interfacial reaction kinetics The basis of the bone-bonding property of bioactive glasses is the chemical reactivity of the glass in the presence of body fluids. The surface reactions lead to the formation of a HCA layer and as a result of this sequence of reactions, bonding of implant to tissue occurs. Hench and West 1 proposed a complex process for the formation of apatite layer involving five reac- tion stages (listed below). The literature for stages 1 and 2 is quite extensive, 17–19 whereas the study of stages 3 to 5 is limited but conclusive. Correspondence to: P. Saravanapavan; e-mail: p.pavan@ ic.ac.uk © 2003 Wiley Periodicals, Inc.