Open Journal of Blood Diseases, 2023, 13, 24-32 https://www.scirp.org/journal/ojbd ISSN Online: 2164-3199 ISSN Print: 2164-3180 DOI: 10.4236/ojbd.2023.131004 Feb. 24, 2023 24 Open Journal of Blood Diseases Glucose-6-Phosphate Dehydrogenase Deficiency: Difficulties in Diagnosis at the Souro Sanou University Hospital, Burkina Faso Salifo Sawadogo 1* , Koumpingnin Nébié 2 , Jerôme Kouldiati 3 , Catherine Traoré 1 , Salam Sawadogo 2 , Aimée S. Kissou 1 , Eléonore Kafando 4 1 Souro Sanou University Hospital, Bobo-Dioulasso, Burkina Faso 2 National Blood Transfusion Center, Ouagadougou, Burkina Faso 3 Yalgado Ouédraogo University Hospital, Ouagadougou, Burkina Faso 4 Charles De Gaule University Pediatric Hospital, Ouagadougou, Burkina Faso Abstract Glucose-6-phosphate dehydrogenase deficiency is the most common enzy- mopathy worldwide. The precise prevalence of G6PD is unknown in Burkina Faso. The objective of the study was to describe the difficulties to diagnose this disease at the Souro Sanou University hospital (CHUSS) in Bobo-Dioulasso. It involved five patients comprising one child with homozygous SS sickle cell disease, one adolescent screened following a family investigation, and three adults including a man and two women. Blood smear stained with May Grunwald Giemsa was performed to look for specific signs of G6PD-deficient red blood cell and brilliant cresyl Blue for Heinz Bodies. A microscope Olympus BX53 equipped with a Camera (XC10) and connected to a comput- er was used to read blood smears and capture images. Genes sequencing by Sanger method were performed in a specialized laboratory in molecular ge- netics. For each analysis, the protocol and instructions of the equipment and reagent manufacturer were applied. Of the five patients, three had anemia and only one had hyperreticulocytosis. Two patients had biological signs of hemolysis and one patient had an elevated CRP. Blood smear stained with MGG and cresyl blue showed specific signs of G6PD-deficient red blood cells and Heinz bodies in all patients. Biochemical analysis and molecular typing confirmed G6PD deficiency. The presence of G6PD-deficient red blood cells in the blood smear guides the diagnosis of G6PD deficiency. The diagnosis is biochemical and is based on the combined measurement of G6PD plus pyru- vate kinase and/or hexokinase. How to cite this paper: Sawadogo, S., Nébié, K., Kouldiati, J., Traoré, C., Sawa- dogo, S., Kissou, A.S. and Kafando, E. (2023) Glucose-6-Phosphate Dehydroge- nase Deficiency: Difficulties in Diagnosis at the Souro Sanou University Hospital, Bur- kina Faso. Open Journal of Blood Diseases, 13, 24-32. https://doi.org/10.4236/ojbd.2023.131004 Received: November 23, 2022 Accepted: February 21, 2023 Published: February 24, 2023 Copyright © 2023 by author(s) and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ Open Access