Research Article Effects of Omega-3-Rich Pork Lard on Serum Lipid Profile and Gut Microbiome in C57BL/6NJ Mice Anantawat Koontanatechanon, 1,2 Manoosak Wongphatcharachai, 2 Nutthawan Nonthabenjawan, 2 Pichaya Jariyahatthakij, 2 Thanchanok Khorporn, 2 Wanpeuch Parnsen, 2 Benjawan Keattisin, 3 Pattarin Leksrisompong, 3 Pairat Srichana, 2 Sattrachai Prasopdee, 1 Sittiruk Roytrakul, 4 Kusuma Sriyakul, 1 Veerachai Thitapakorn , 1 and Kammal Kumar Pawa 1 1 Chulabhorn International College of Medicine, Thammasat University, Pathumthani, Thailand 2 Feed Technology Office, Charoen Pokphand Foods Public Company Limited (CPF), Bangkok, Thailand 3 CPF Food Research & Development Center, Charoen Pokphand Foods Public Company Limited (CPF), Bangkok, Thailand 4 National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology for Development Agency (NSTDA), Pathumthani, Thailand Correspondence should be addressed to Veerachai Thitapakorn; veebkk@gmail.com and Kammal Kumar Pawa; kammalkumarpawa@gmail.com Received 1 April 2022; Revised 24 October 2022; Accepted 29 October 2022; Published 22 November 2022 Academic Editor: Ivan Salmerón Copyright © 2022 Anantawat Koontanatechanon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background and Aims. Hyperlipidemia is a risk factor for cardiovascular diseases. This study is aimed at investigating the effects of consuming omega-3-rich pork lard on the serum lipid profile and gut microbiome of the mice model. Methods and Results. We divided 23 C57BL/6NJ males (16-week-old) into 3 groups, and each group received either a control diet, a high-fat diet of coconut oil (coconut oil), or a high-fat diet of omega-3-rich pork lard (omega lard) for 28 days. Thereafter, fasting serum lipids and fecal microbiomes were analyzed. The serum cholesterol, triglyceride, and LDL levels of the omega lard-treated group were significantly reduced compared to the coconut oil-treated group (P <0:05). However, the microbiome analysis revealed a significant increase in the abundance of Lachnospiraceae in the omega lard-treated group compared to the coconut oil-treated group (P <0:05). Furthermore, Spearman’s correlation analysis revealed that the increased serum lipid content was positively correlated with the abundance of Bacteroidaceae (P <0:05) and negatively correlated with the abundance of Lachnospiraceae (P <0:05). Conclusions. These findings suggested that omega-3-rich pork lard altered the serum lipid profile and gut microbiome in the mice model. Practical Application. The excellent protection offered by omega-3-rich pork lard against hyperlipidemia indicated that pork lard could be used as alternative cooking oil for health-conscious individuals. It could also be introduced as a functional ingredient for patients with hyperlipidemia. 1. Introduction Reports suggest an association between dietary nutrition and health or immunity, especially an association between dete- riorated health and the Western diet, which contains high dietary fats [1]. Owing to the high content of omega-6 fatty acids and low proportion of vegetables and fibers in the Western diet, the prevalence of obesity and other metabolic diseases, including cardiovascular disease (CVDs) and non- alcoholic fatty liver disease (NAFLD), has increased signifi- cantly in the past years [2]. However, replacing it with a beneficial fatty acid source can potentially ameliorate a few of the metabolic diseases [3]. Several studies evaluated the health benefits of omega-3 supplements, including the improvement of metabolic parameters and cardioprotective effects in an animal model Hindawi International Journal of Food Science Volume 2022, Article ID 9269968, 14 pages https://doi.org/10.1155/2022/9269968