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Original Article
Oncol Res Treat 2015;38:24–27
DOI: 10.1159/000370341
The Preoperative Neutrophil/Lymphocyte Ratio Does
not Correlate with the 21-Gene Recurrence Score
in Estrogen Receptor-Positive Breast Cancer Patients
Tal Grenader
a, b
Yevgeni Plotkin
c
David B. Geffen
c, d
a
Institute of Oncology, Shaare Zedek Medical Center, Jerusalem, Israel;
b
University College London, United Kingdom;
c
Soroka University Medical Center, Beer Sheva, Israel;
d
Ben-Gurion University of the Negev, Beer Sheva, Israel
Introduction
Breast cancer (BC) is the most prevalent malignancy worldwide
and the second leading cause of cancer-related death among
women. Roughly 75% of these tumors are hormone receptor (HR)
positive. In patients with estrogen receptor (ER)-positive, early-
stage BC, the 21-gene recurrence score (RS) assay (OncotypeDX
TM
;
Genomic Health Inc., Redwood City, CA, USA) is widely employed
to help determine appropriate candidates for adjuvant chemother-
apy [1].
The RS ranges from 0 to 100 and constitutes a measure of the
risk of distant relapse within 10 years for patients with ER-positive,
node-negative BC, treated with adjuvant tamoxifen. It also predicts
the individualized benefit of hormonal therapy and chemotherapy
[1]. Currently, evidence suggests that the RS is prognostic for pa-
tients with ER-positive BC with node-positive disease as well [2].
This test is relevant in nearly half of all newly diagnosed BC pa-
tients. Therefore, its wide use can represent significant costs to
healthcare systems, considering that most of the patients have
early-stage and ER-positive BC.
The importance of systemic inflammation in promoting car-
cinogenesis and tumor progression is well recognized. Inflamma-
tion in the tumor microenvironment plays an important role in the
proliferation and survival of malignant cells and was found to
modulate tumor function, with both anti- and pro-tumor effects,
in many solid tumors. Recent data suggest that systemic inflamma-
tion contributes to the development and progression of BC. In-
flamed adipose tissue within the breast is associated with elevated
levels of pro-inflammatory mediators, enhanced expression of aro-
matase (the rate-limiting enzyme for estrogen biosynthesis), and
increased ER-α-dependent gene expression [3]. Numerous studies
have established that elevated inflammatory markers, such as C-
Keywords
Breast cancer · Estrogen receptor · Gene assay ·
Neutrophil/lymphocyte ratio · OncotypeDX
TM
·
Recurrence score
Summary
Aims: The aim of this study was to examine the correla-
tion, if any, between the preoperative neutrophil/lym-
phocyte ratio (NLR) and the OncotypeDX
TM
21-gene re-
currence score (RS) in patients with early-stage estrogen
receptor (ER)-positive breast cancer (BC). Materials and
Methods: We retrospectively reviewed the medical re-
cords of patients diagnosed with primary ER-positive BC
who were referred for the RS assay. The correlation be-
tween the preoperative NLR and the RS was analyzed.
Results: For the 242 patients with sufficient data for anal-
ysis, the median age at diagnosis was 59.5 years. The
tumor size ranged from 0.50 to 5.50 cm, with a mean size
of 1.8 cm; 73.2% of the tumors were < 2 cm in size. Most
of the tumors (66.3%) were of grade 2; the rest was
nearly equally divided between grades 1 and 3. The test
results for the progesterone receptor (PR) were positive
in 86.6% of the cases. Lymph node metastases were pre-
sent in 22.3% of the patients. The median RS was 18
(range 0–60) and the mean NLR value was 2.11 (range
0.49–7.49). We found no significant correlation between
the NLR and the RS. Conclusion: Our data suggest that
the preoperative NLR does not predict the 21-gene RS in
patients with early-stage hormone-sensitive BC.
Received: June 12, 2014
Accepted: November 19, 2014
Published online: January 12, 2015
Tal Grenader, MD
Department of Oncology
Shaare Zedek Medical Center
P.O. Box 3235, 91031 Jerusalem, Israel
talgrenader65@hotmail.com
© 2015 S. Karger GmbH, Freiburg
2296-5270/15/0381-0024$39.50/0
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