_____________________________________________________________________________________________________ *Corresponding author: E-mail: etitigwun@yahoo.com; J. Adv. Biol. Biotechnol., vol. 26, no. 3, pp. 22-32, 2023 Journal of Advances in Biology & Biotechnology Volume 26, Issue 3, Page 22-32, 2023; Article no.JABB.96600 ISSN: 2394-1081 Analysis of Food Items and Diet Preference in the Stomach of Synodontis obesus (Boulenger, 1898: Siluriformes, Mochokidae) in the Lower Cross River, Nigeria John Etitigwun Ukpatu a* and Idopiseabasi Ekpe Asuquo b a Department of Fisheries and Aquaculture, Faculty of Agriculture, Akwa Ibom State University, Obio Akpa Campus, Nigeria. b Department of Fisheries and Aquaculture, Akwa Ibom State University, Nigeria. Authors’ contributions This work was carried out in collaboration between both authors. Both authors read and approved the final manuscript. Article Information DOI: 10.9734/JABB/2023/v26i3625 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/96600 Received: 01/02/2023 Accepted: 13/02/2023 Published: 15/05/2023 ABSTRACT Analysis of stomach contents of Synodontis obesus (BOULENGER, 1898: SILURIFORMES, MOCHOKIDAE) in the Lower Cross River, Nigeria was carried out with standard methods to assess the food items and diet preference of the S. obesus. A total of 160 individuals of S. obesus (Mochokidae) were collected from October 2022 - January, 2023 at Akan Obio Uraun fishing port in Itu Local Government Area, Lower Cross River, Nigeria from local fishers and used for the study. Analysis of the fullness of stomach showed that 84.4% had food contents while 15.60% were empty stomachs. Samples with one quarter (25%) stomach fullness had the highest value of 28.13% while samples with 100% stomach fullness (18.75%) were more than those with half (50%) stomach Original Research Article