DEVELOPMENTAL MEDICO-LIFE-SCIENCES ISSN (P): 3007-2786, (E): 3007-2794 https://dmlsjournal.com/index.php/January2024/issue/view/april-2024 Page 8 of 19 Persistence And Efficacy of Maternal Covid-19 Vaccine-Induced Antibodies in Human Milk: A Comprehensive Meta-Analysis Neha Fatima 1* , Syeda Nawal Fatima 2 , Nisha Noor 1 , Marium Khan 2 , Khadija Yahya 2 , Mustafa Rizwan 1 , Mamoona Shuja 3 , Muhammad Nasir Shahbaz 4 1- Pharmacy Department, Lahore Medical & Dental College (LMDC), Lahore, Pakistan. 2- BDS, Lahore Medical & Dental College (LMDC), Lahore, Pakistan. 3- MBBS, Al-Aleem Medical College, Lahore, Pakistan. 4- Institute of Molecular Biology & Biotechnology (IMBB), CRIMM, The University of Lahore, Lahore, Pakistan. *Corresponding Author: Dr. Neha Fatima, Email: Nuziha72@gmail.com , Cell: +92-3234904762 © The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/public domain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Abstract Background: The ongoing global health challenge posed by the COVID-19 pandemic has necessitated a thorough examination of vaccine efficacy, particularly the transfer of vaccine-induced immunity to infants via human milk. This meta-analysis aims to explore the persistence and efficacy of COVID-19 vaccine-induced antibodies in human milk, offering insights into passive immunity transfer to breastfeeding infants. Methods: We conducted a systematic review and meta-analysis of peer-reviewed studies published from January 2021 to December 2023, extracted from databases such as PubMed, Scopus, and Web of Science. Our focus was on studies measuring SARS-CoV-2-specific antibodies (IgA and IgG) in human milk following maternal COVID- 19 vaccination. Results: Fifteen studies involving 1,200 lactating mothers who received mRNA COVID-19 vaccines were included. The analysis highlighted a consistent presence of SARS-CoV-2-specific IgA and IgG antibodies in human milk, persisting for up to six months post-vaccination. Conclusion: The results highlight the noteworthy transmission of COVID-19 antibodies via human milk, suggesting an extended duration of possible protection for neonates against SARS-CoV-2 infection. This argues in Favor of immunizing nursing mothers as a preemptive public health strategy to provide their children passive immunity. Keywords: COVID-19; SARS-CoV-2; antibodies; human milk; maternal vaccination; passive immunity; breastfeeding; IgA; IgG; meta-analysis. Received: 05/05/2024 Revised: 06/05/2024 Accepted: 06/05/2024 Published: 28/05/2024