National Journal of Community Medicine│Volume 7│Issue 4│Apr 2016 Page 278 ORIGINAL ARTICLE pISSN 0976 3325│eISSN 2229 6816 Open Access Article www.njcmindia.org A STUDY OF COLONIZATION WITH ESBL AND AMPC E COLI IN GUT OF PATIENTS OF TERTIARY CARE HOSPITAL, AHMEDABAD Sachin M Patel 1 , Nidhi Sood 2 , Parul Patel 1 Financial Support: None declared Conflict of interest: None declared Copy right: The Journal retains the copyrights of this article. However, reproduction of this article in the part or total in any form is permissible with due acknowledgement of the source. How to cite this article: Patel SM, Sood N, Patel P. A Study of Colonization with ESBL and Ampc E Coli in Gut of Patients of Tertiary Care Hospital, Ahmedabad. Ntl J Commu- nity Med 2016; 7(4):278-280. Author’s Affiliation: 1 Assistant Professor; 2 Professor & Head, Microbiology Department, GMERS Medical College, Sola, Ah- medabad, India Correspondence: Dr. Sachin M. Patel drsachinpatel1982@gmail.com Date of Submission: 18-12-15 Date of Acceptance: 10-04-16 Date of Publication: 30-04-16 ABSTRACT Background: Higher prevalence of ESBL (Extended spectrum beta- lacamase)-producing E. coli in fecal carriage has been reported in the nosocomial setting than in the community due to high levels of antibiotic consumption. This study is to know the prevalence of ESBL producing E.coli in stool samples of Hospitalized patient with non GIT complains. Material & Methods: A total 300 Stool samples of the patients with non GIT complains admitted in General Hospital, Sola, Ahmadabad were collected for study between December 2012 to March 2013. All samples were tested for routine stool microscopy as well as culture and sensitivity according to NCCLS guideline. Result: Of 100 samples showing E.coli out of 300 samples, 43% show ESBLs producing E.coli and Amp C producing E.coli show in 26% cases. While 31% was normal E.coli without ESBLs B- lactamase and AmpC B lactamase. Commonest age group is 0-15 years. Conclusion: Our normal gut flora is highly replaced by the ESBLs/AmpC producing E.coli, though they are non pathogenic organism. A threatening epidemiological problem is the dissemi- nation of ESBL-producing organisms to healthy people in the community, which might depend on the frequency of ESBL fecal carriage as well as on the presence of ESBL producing organisms in the food chain. Key words: ESBL, E.coli, AmpC INTRODUCTION Multidrug resistance is increasingly seen in many Gram-negative bacteria as a result of widespread use of various antibiotics. 1,2 . Extended spectrum β- Lactamase (ESBLs) are enzymes that commonly mediate resistance to β-Lactam antimicrobial drugs in Gram-negative bacteria 3 and are most com- monly found in Escherichia coli and Klebsiella pneumoniae. 1, 2 ESBL-producing organisms have been widely re- ported in many countries. 4 These enzymes are typically plasmid-mediated. . 4 ESBLs are often encoded by genes located on large plasmids and these also carry genes for resistance to other antim- icrobial agents such as Aminoglycosides, Trimethoprim, Sulphonamides, Tetracycline, Chloramphenicol and Fluoroquinolones. 4 They are not active against cephamycins or carbapenems, and are highly susceptible in vitro to inhibition by lactamase inhibitors, such as clavulanic acid 5. Higher prevalence of ESBL-producing E. coli in fecal carriage has been reported in the nosocomial setting than in the community. 6 Patients of medi- cal units with high levels of antibiotic consumption have also shown higher rates of ESBL colonization . 7 Patients with community infections and members of their households represent a reservoir for ESBL producers, increasing the dispersal of resistance in healthy people. 7 Rates of colonization by extended-