© 2012 Syed Asad B, Iqbal MM, Kiranmai M & Ibrahim M. This is a research/review paper, distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License http://creativecommons.org/licenses/by-nc/3.0/), permitting all non commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Global Journal of Medical Research Volume 12 Issue 6 Version 1.0 Year 2012 Type: Double Blind Peer Reviewed International Research Journal Publisher: Global Journals Inc. (USA) Online ISSN: 2249-4618 Print ISSN:0975-5888 Hepatoprotective Activity of Phyllanthus Amarus Seeds Extracts in CCl4 Treated Rats: In Vitro & In Vivo By Syed Asad B, Iqbal MM, Kiranmai M & Ibrahim M Asian Institute of Advance Scientific & Pharmaceutical Research, India Abstract - Aim - To study the hepatoprotective activity of Phyllanthus amarus seeds extracts in CCl4 treated rats. Methods - The crushed and dried seeds of Phyllanthus amarus were divided into two parts; one part was extracted successively with petroleum ether, benzene, chloroform and finally with methanol by soxhlet apparatus and concentrated by rotary vacuum. The other part is extracted by cold maceration process for aqueous extraction. The extracts were used for In-vitro and In-vivo studies to analyze the reparative activity of liver injury due to CCl4 in rats. Results - In-vitro models like Reducing power, Superoxide anion scavenging activity, Hydroxyl radical scavenging activity and Nitric oxide radical scavenging activity were carried out with methanolic extract of Phyllanthus amarus seeds for its antioxidant properties. A protective activity could be demonstrated in the CCl4 induced liver damage in rats. In-vivo methanolic and aqueous extracts of the seeds of Phyllanthus amarus 250mg/kg were found to have protective properties in rats with CCl4 induced liver damage as judged from serum biochemical enzyme marker activities and histopathological studies.. Keywords : Hepatoprotective activity, Phyllanthus amarus seeds, CCl4 and Methanolic extract. HepatoprotectiveActivityofPhyllanthusAmarusSeedsExtractsinCCl4TreatedRatsInVitro InVivo Strictly as per the compliance and regulations of: GJMR-G Classification : NLMC Code: WI 740,QY 60.R6, W 20.55.A5