Tissue Antigens ISSN 0001-2815 BRIEF COMMUNICATION Study of association of CTLA4 gene variants to non-anterior uveitis D. A. Leon Rodriguez 1† , A. Serrano Lopera 1† , M. Cordero-Coma 2 , A. Márquez 1,3 , A. Fonollosa 4 , I. Ruiz-Arruza 5 , J. L. Callejas 3 , J. L. García Serrano 6 , D. Díaz Valle 7 , E. Pato 8 , J. Cañal 9 , M. J. del Rio 10 , M. J. Capella 11 , A. Blanco 12 , J. L. Olea 13 , Y. Cordero 14 , J. M. Martín-Villa 15 , M. B. Gorroño-Echebarría 16 , B. Molins 17 , A. Adán 17 & J. Martin 1 1 Instituto de Parasitología y Biomedicina López-Neyra, IPBLN-CSIC, P.T.S, Granada, Spain 2 Ophthalmology Department, Hospital de León, IBIOMED, Universidad de León, León, Spain 3 Systemic Autoimmune Diseases Unit, Hospital Clínico San Cecilio, Granada, Spain 4 Ophthalmology Department, BioCruces Health Research Institute, Hospital Universitario Cruces, Barakaldo, Spain 5 Autoimmune Diseases Research Unit, Internal Medicine Department, BioCruces Health Research Institute, Hospital Universitario Cruces, Barakaldo, Spain 6 Ophthalmology Department, Hospital Clínico San Cecilio, Granada, Spain 7 Ophthalmology Department, Hospital Clínico San Carlos, Madrid, Spain 8 Rheumatology Department, Hospital Clínico San Carlos, Madrid, Spain 9 Ophthalmology Department, Hospital Marqués de Valdecilla, IDIVAL, Santander, Spain 10 Ophthalmology Department, Hospital Carlos Haya, Málaga, Spain 11 Institut Universitari Barraquer, Universitat Autònoma de Barcelona, Barcelona, Spain 12 Ophthalmology Department, Hospital Donostia, San Sebastián, Spain 13 Ophthalmology Department, Hospital Son Espases, Palma de Mallorca, Spain 14 Ophthalmology Department, Hospital Universitario Rio Hortega, Valladolid, Spain 15 Immunology Department, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, Spain 16 Ophthalmology Department, Hospital Universitario Principe de Asturias, Alcalá de Henares, Spain 17 Ophthalmology Department, Hospital Clinic, Barcelona, Spain Key words CTLA4; rs5742909 and rs231775; rs733618; single nucleotide polymorphisms; uveitis Correspondence Javier Martin Instituto de Parasitología y Biomedicina López-Neyra IPBLN-CSIC P.T. Ciencias de la Salud Avenida del Conocimiento s/n 18016 Granada Spain Tel: +34 958 18 16 21 Fax: +34 958 18 16 33 e-mail: martin@ipb.csic.es Received 8 June 2015; revised 31 July 2015; accepted 25 August 2015 doi: 10.1111/tan.12668 Abstract This study was undertaken to investigate the possible genetic association of functional CTLA4 polymorphisms with susceptibility to non-anterior uveitis. Four hundred and seventeen patients with endogenous non-anterior uveitis and 1517 healthy controls of Spanish Caucasian origin were genotyped for the CTLA4 polymorphisms rs733618, rs5742909 and rs231775, using predesigned TaqMan © allele discrimination assays. PLINK software was used for the statistical analyses. No significant associations between the CTLA4 polymorphisms and susceptibility to global non-anterior uveitis were found. It was also the case when the potential association of these genetic variants with the anatomical localization of the disease, such as intermediate, posterior or panuveitis, was assessed. Our results do not support a relevant role of these CTLA4 polymorphisms in the non-anterior uveitis genetic predisposition. Uveitis is defined as the inflammation of the uvea, the part of the middle eye that includes the iris, ciliary body and choroid, three highly vascularized and pigmented areas (1). According † Both authors contributed equally to this work. to the localization of inflammation, uveitis could be classi- fied as: anterior, involving iris or ciliary body; intermediate, involving the vitreous, peripheral retina, pars plana or ciliary body; posterior, affecting choroid or by extension of the retina and panuveitis, which is referred to inflammation localized in two or more segments of the uveal tract (2). Additionally, © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd 373 Tissue Antigens, 2015, 86, 373–376