Journal of Pharmaceutical Negative Results ¦ Volume 13 ¦ Special Issue 8 ¦ 2022 2026 Comparative Standardization Study of Ayurvedic Formulations of Gokshuradi Churna C. Kavitha 1* , Dr.C.N. Nalini 2 , Dr.R. Radha 3 , Dr.T. Purushoth Prabhu 4 , Dr.R. Vijaya Bharathi 5 1* Associate Professor, Department of Pharmacognosy, C.L Baid Metha College of Pharmacy, Affiliated to the Tamilnadu DR.M.G.R Medical University, Chennai, Tamil Nadu, India. E-mail: kavithacmpharm@gmail.com 2 Department of Pharmaceutical Analysis, C.L Baid Metha College of Pharmacy, Affiliated to the Tamilnadu DR.M.G.R Medical University, Chennai, Tamil Nadu, India. 3 Department of Pharmacognosy, College of Pharmacy, Madras Medical College, Affiliated to the Tamilnadu DR.M.G.R Medical University, Chennai, Tamil Nadu India. 4 Department of Pharmacognosy, C.L Baid Metha College of Pharmacy, Affiliated to the Tamilnadu DR.M.G.R Medical University, Chennai, Tamil Nadu, India. 5 Department of Pharmacognosy, College of Pharmacy, Madras Medical College, Affiliated to the Tamilnadu DR.M.G.R Medical University, Chennai, Tamil Nadu, India. The Tamilnadu Dr.M.G.R. Medical University, Chennai, Tamil Nadu, India. Background: The knowledge of plant based medicines was gradually developed by the primeval persons and was passed on to generations by trial and error basis without proper scientific evaluation, safety and toxicological studies. The discovery and acceptance of natural therapies in the past decades has been increasing in both developing and developed countries. Consumers buy herbal products without a prescription through online. Thus standardization is required with systematic approach and well-designed methodologies to maintain uniformity between batches during production of herbal products. The present work aims for the comparative standardization of the marketed formulation and two in-house prepared Gokshuradi Churna. It is an ayurvedic formulation prescribed for inflammation, arthritis, and other joint disorders. Gokshuradi Churna consists of powdered mixture of herbal drugs in equal proportions: the fruits of Tribulus terrestris, the fruits of Terminalia chebula, the root of Boerhaavia diffusa, the wood of Cedrus deodara, the rhizome of Zingiber officinale, and the bulb of Allium sativum. Materials and Methods: The churna was standardized on the basis of the parameters like organoleptic characters, physical characters, physicochemical properties, phytochemical screening. Results: The parameters studied were within standard limits and the quality of the products were established. The results obtained may be considered as reference standards for developing standard formulation. Conclusion: From preliminary studies, it confirms that In house and marketed formulations of Gokshuradi Churna is safe for therapeutic use. Further studies has to be done to confirm the pharmacological activities of the churna. Keywords: Standardization, Traditional Medicine, Physicochemical Parameter, Phytochemical Analysis, Gokshuradi Churna, Quality Control. DOI: 10.47750/pnr.2022.13.S08.248 INTRODUCTION The use of herbs as medicine is the oldest form of healthcare known to mankind and is practiced in all cultures throughout history. Herbal medicines are plants and plant derived products used for centuries for their therapeutic or medicinal properties. They are made from any part of the plants but commonly from leaves, roots, bark seeds, and flowers. They are eaten, swallowed, drunk, inhaled, or applied topically to the skin Herbal products often contain a variety of naturally- occurring biochemical’s from plants, many of which contribute to the plant’s medicinal effects. Herbal medicines are obtained from variable sources. So the quality of herbal medicine is important to get the desirable effects. Standardization of herbal medicines is the process of prescribing a set of standards or inherent characteristics, constant parameters, definitive qualitative and quantitative values that carry an assurance of quality, efficacy, safety and reprehensibility. Specific standards are worked out by experimentation and observations, which would lead to the process of prescribing a set of characteristics exhibited by the particular herbal medicine. Hence standardization is a tool in the quality control process (1). Need for Standardization of Herbal Medicine Herbal medicines are associated with several problems. 1. Herbal drugs are usually mixtures of many active constituents and in most cases it is unknown. 2. Selective analytical methods or reference standards may not be available commercially. 3. The source and quality of the raw material are chemically and naturally variable. 4. The methods of harvesting, drying, storage, transportation, and