S120 Abstracts / Journal of Biotechnology 185S (2014) S37–S125 fatty acids were found in all strains. Physiochemical characteri- zation of rhamnolipids was evaluated by the emulsiﬁcation test, oil displacement test and phenanthrene solubilization. The com- position and properties of the produced rhamnolipids using above mentioned strains were different, which allows preparing appro- priate mixtures depending on the intended applications. We have also demonstrated that all the applied producers allow the biore- actor cultivation in mixtures (ternary or dual cultures) while maintaining stable rhamnolipid production. It was conﬁrmed that this is another way to prepare rhamnolipids of desired properties. http://dx.doi.org/10.1016/j.jbiotec.2014.07.409 Cyanobacterial strains, isolated from extreme conditions sources of Kazakhstan – Producers of biodiesel Bolatkhan Zayadan 1,∗ , Fariza Sarsekeyeva 1 , Kirill Mironov 2 , Aizhan Userbayeva 1 , Dmitry Los 2 1 Department of Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan 2 Laboratory of Intracellular Regulation, Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia E-mail address: zbolatkhan@mail.ru (B. Zayadan). In recent years a systematic and orderly mastering new cultures of cyanobacteria for further practical use in biotechnology. In this regard, the aim of this work was the isolation, identiﬁcation and determination of fatty acid composition of new strains of cyanobac- teria from extreme conditions sources of Kazakhstan. Samples were isolated from lake Issyk (13 ◦ C), hot spring Turgen (45 ◦ C) and salinity lake Balkhash (salinity 4 g/l). The standard techniques of cultivation of phototrophic microorganisms were used. Fatty acids were derived using methyl esters and separated by GC–MS Ali- gent 7890GC. The results showed that 3 different strains were obtained: sp2 from Issyk, sp1 from Turgen and sp1 from Balkhash. Morphological characteristics and 16S rDNA gene were identiﬁed those strains as Synechococcus elongatus, Cyanobacterium aponinum and Cyanobacterium stanieri. Strain Synechococcus elongatus sp.I2 synthesized saturated 26.9% (generally palmitic) and unsaturated 73.1% (oleic, lenoic and lenoleic) FA. In Cyanobacterium aponinum sp.T1 strain advantageously synthesized saturated 62.8% (myris- tic and palmitic) and monoenoic 37.1% (palmitoleic) FA. Cells of strain Cyanobacterium stanieri sp.B1 characterized by accumula- tion mainly of saturated and monounsaturated FA thus dominated myristic (30.1%) and palmitoleic (39.7%) acids. All isolates based on the composition of the FA can be used as producers for the production of biodiesel. http://dx.doi.org/10.1016/j.jbiotec.2014.07.410 Environmental beneﬁts of seaweed biomass as a bioenergy feedstock Seong Rin Lim 1,∗ , Hye Rim Park 2 , Kyung A. Jung 2 , Ji Hye Jo 3 , Jong Moon Park 2 1 Department of Environmental Engineering, College of Engineering, Kangwon National University, Chuncheon, Republic of Korea 2 School of Environmental Science and Engineering, and Division of Advanced Nuclear Engineering, POSTECH, Republic of Korea 3 Division of Resource Circulation, Korea Environment Institute, Republic of Korea E-mail address: srlim@kangwon.ac.kr (S.R. Lim). Bioethanol is produced from a diverse range of biomasses. Recently, many interests have been taken in marine seaweed as a new bioethanol feedstock. Thus, the objective of this study is to quantify the bioenergy potential of marine seaweed. We estimated poten- tial ethanol yields of marine biomass. Red seaweeds have higher ethanol yields than brown seaweeds, even though realistic produc- tivity of brown seaweeds was higher than for red seaweeds, due to advanced farming technology. For carbon-neutral ability, marine seaweeds have negligible carbon emissions in biomass production, compared to terrestrial biomasses, due to the difference for land use. Seaweed production has higher carbon sequestration poten- tial than for terrestrial crops. The seaweed-based bioethanol does not require irrigation water in farming and can reduce the use of water in the fermentation process by using seawater. Marine sea- weeds do not need fertilizers and pesticides in farming. Also, the seaweed-based ethanol helps establish the nutrient (nitrogen and phosphorus) cycles in nature by returning the nutrients in the sea- weed fermentation residue to terrestrial ecosystems, due to high nutrient composition. Therefore, this study can contribute to exam- ining the potential of marine seaweed as a sustainable bioenergy feedstock. http://dx.doi.org/10.1016/j.jbiotec.2014.07.411 Synthesis of methyl ester sulfonate surfactant from palm oil methyl ester by using UV or ozone as an initiator Hattaya Tulathammakit ∗ , Boonyarach Kitiyanan The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok 10330, Thailand E-mail address: hattaya.tula@gmail.com (H. Tulathammakit). Methyl ester sulfonate (MES) is a potential anionic surfac- tant + derived from palm oil methyl ester. MES shows several advantages, such as excellent cleaning properties, good biodegrad- ability, and tolerance to hard water. Although alpha methyl ester sulfonate (-MES) is commercially available, it still has some draw- backs, such as disalt formation and low water solubility. In order to overcome these drawbacks, MES was synthesized via sulfoxidation by using different initiators. Ozone is one of the most interesting ini- tiators because of its highly oxidizing power, which can react with organic compound rapidly under mild conditions. In this work, the starting methyl ester was comprised of 36% C16 and 64% C18. The reaction was performed at 40 ◦ C under atmospheric pressure in a photochemical reactor with 16 UV lamps (253.7 nm). The outlet product was transferred to separation and puriﬁcation processes by using liquid extraction techniques. The structure of MES was conﬁrmed by FT-IR and ESI-MS. When reaction time was ﬁxed at