Research Article
Phenolic Acids (Gallic and Tannic Acids) Modulate Antioxidant
Status and Cisplatin Induced Nephrotoxicity in Rats
Seun F. Akomolafe,
1
Ayodele J. Akinyemi,
2
and Scholarstical O. Anadozie
2
1
Department of Biochemistry, Ekiti State University, P.M.B 5363, Ado Ekiti, Nigeria
2
Department of Biochemistry, Afe Babalola University, P.M.B 5454, Ado Ekiti, Nigeria
Correspondence should be addressed to Seun F. Akomolafe; purposefulseun@yahoo.co.uk
Received 21 March 2014; Revised 21 May 2014; Accepted 2 June 2014; Published 11 August 2014
Academic Editor: Ottavio Giampietro
Copyright © 2014 Seun F. Akomolafe et al. is is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly
cited.
Cisplatin (cis-diamminedichloroplatinum (II) or CDDP), used in the treatment of many solid-tissue cancers, has its chief side-
effect in nephrotoxicity. Hence, this study sought to investigate and compare the protective effect of gallic acid (GA) and tannic
acid (TA) against cisplatin induced nephrotoxicity in rats. e rats were given a prophylactic treatment of GA and TA orally at a
dose of 20 and 40mg/kg body weight for 7 consecutive days before the administration of a single intraperitoneal (i.p.) injection
of cisplatin (CP) at 7.5mg/kg bwt. e protective effects of both GA and TA on CP induced nephrotoxicity were investigated by
assaying renal function, oxidative stress biomarkers, and histopathological examination of kidney architecture. A single dose of
cisplatin (7.5 mg/kg bwt) injected i.p. caused a significant increase in some biomarkers of renal function (creatinine, uric acid, and
urea levels), with a marked elevation in malondialdehyde (MDA) content accompanied by a significant ( < 0.05) decrease in
reduced glutathione (GSH) content (103.27%) of kidney tissue as compared to control group. Furthermore, a significant ( < 0.05)
reduction in kidney antioxidant enzymes (SOD, catalase, GPx, and GST) activity was observed. However, pretreatment with oral
administration of tannic acid and gallic acid at a dose of 20 and 40mg/kg body weight, respectively, for 7 days prior to cisplatin
administration reduced histological renal damage and suppressed the generation of ROS, lipid peroxidation, and oxidative stress in
kidney tissues. ese results indicate that both gallic and tannic acids could serve as a preventive strategy against cisplatin induced
nephrotoxicity.
1. Introduction
e use of chemotherapy in the treatment of cancer has
opened new possibilities for improvement of the quality of life
of cancer patients. Despite its success, treatment with some of
the most effective anticancer drugs shows a number of symp-
toms of direct toxicity [1]. In recent years, the mechanism
of cisplatin (cis-diamminedichloroplatinum (II) or CDDP)
induced nephrotoxicity has gradually been elucidated [2].
Studies have shown an increase in lipid peroxides in the
renal tissue of CDDP-administered animals [3], a decrease
in reduced glutathione levels [1], and the induction of
metallothionein [4], an antioxidant. ese changes have been
considered to result from the generation of reactive oxygen
species (ROS). Studies using chemiluminescence or electron
spin resonance (ESR) have shown that CDDP generates OH
radical [5, 6].
Nephrotoxicity involves kidney damage or dysfunction
arising from direct or indirect exposure to drugs and
industrial or environmental chemicals. Cisplatin ((cis-
diamminedichloroplatinum (II) or CDDP)), an anti-
neoplastic drug have been reported to induce nephrotoxicity
[7]. e kidney which is the major route of cisplatin excretion
also accumulates it to a greater degree than other organs
[6, 8]. Oxidative stress, inflammation, and apoptosis are some
of the mechanisms already established to explain cisplatin
induced acute kidney injury [9]. A number of strategies have
been proposed for the prevention/management of cisplatin
induced nephrotoxicity, since there is no specific treatment,
with the use of some synthetic drugs which have been
Hindawi Publishing Corporation
International Scholarly Research Notices
Volume 2014, Article ID 984709, 8 pages
http://dx.doi.org/10.1155/2014/984709