271 Bulgarian Journal of Agricultural Science, 25 (No 2) 2019, 271–277 Effect of temperature, relative humidity and moisture on aflatoxin contamination of stored maize kernels Francis Collins Muga 1* , Moses Okoth Marenya 2,3** , Tilahun Seyoum Workneh 1*** 1 Department of Bioresources Engineering, School of Engineering, University of KwaZulu-Natal, Private Bag X01, Pietermaritzburg, South Africa 2 Department of Agricultural and Rural Engineering, School of Agriculture, University of Venda, Private Bag X5050, Thohoyandou, South Africa 3 Institute for Agricultural Engineering, Agricultural Research Council, Private Bag X519, Silverton, Pretoria, South Africa * Corresponding author: 215081106@stu.ukzn.ac.za; mugafc@gmail.com Abstract Muga, F. C., Marenya, M. O., & Workneh, T. S. (2019). Effect of temperature, relative humidity and moisture on aflatoxin contamination of stored maize kernels. Bulgarian Journal of Agricultural Science, 25(2), 271–277 This study investigated the aflatoxin contamination of maize kernels for selected temperature, relative humidity and mois- ture content levels. Samples of maize kernels at moisture content levels of 14, 15, 16, 18, and 20% (wb) were inoculated with Aspergillus flavus spores and incubated in a climatic test chamber for ten days at 20°C and 30°C, and relative humidity of 60% and 90%. The results indicated that aflatoxin contamination was significantly (p ≤ 0.05) affected by temperature and relative humidity whereas moisture content had no significant (p > 0.05) effect. Aflatoxin contamination occurred at both 20°C and 30°C. The production of aflatoxin was pronounced at 30°C, ranging between 0.3 μg.kg -1 – 11179.7 μg.kg -1 , compared to 20°C that ranged between 0.8 μg.kg -1 – 733.7 μg.kg -1 . Relative humidity of 90% had higher levels of aflatoxin contamination of between 3.9 μg.kg -1 – 11179.7 μg.kg -1 , while a relative humidity of 60% had levels of aflatoxin contamination of between 0.3 μg.kg -1 – 2.4 μg.kg -1 . The interaction between temperature and relative humidity significantly (p ≤ 0.05) influenced aflatoxin contamination of maize. However, the interaction between temperature and moisture content, moisture content and relative hu- midity, as well as the combined interaction of temperature, moisture content, and relative humidity had no effect on the level of aflatoxin contamination. The results indicate that the level of aflatoxin contamination at a relative humidity of 60% was lower than 5 μg.kg -1 . Consequently, maintaining storage conditions at a relative humidity level of less than 60% results in minimal aflatoxin contamination of maize kernels, thus assuring its safety for consumption. Keywords: Aspergillus flavus; aflatoxin; maize; storage Introduction Sub-Saharan Africa (SSA) experiences severe yield losses in maize. Insufficient post-harvest practices exacer- bate these losses. Losses of up to 50% have been reported across many countries in Africa, most of which are experi- enced during storage (Hodges et al., 2011). Insects and fungi collectively account for more than 50% of grain lost during storage (Udoh et al., 2000). Fungal spoilage of maize is a grave concern due to the mycotoxins associated with it. Maize is very susceptible to fungal degradation, particu- larly Aspergillus and Fusarium which cause aflatoxins and fumonisins respectively (Tefera, 2012). These mycotoxins impact human and animal health and lower the economic