Journal of Pharmaceutical Negative Results ¦ Volume 13 ¦ Special Issue 6 ¦ 2022 1294 A randomised clinico-microbial trial evaluated the use of ozone as a supplement to scaling and root planing in the treatment of chronic periodontitis. Dr. Ratika Shrivastava 1 , Dr. Aishwarya Singh 2 , Dr. Abhilasha Bhawalkar 3 , Dr Ragini Motwani 4 , Dr. Rahul Bachwani 5 , Dr. Sujith Kumar Palleti 6 1 Associate Professor, Department of Periodontology, Rishiraj College of Dental Science & Research Center, Bhopal, MP 2 Associate Professor, Department of Dentistry, Rajmata Shrimati Devendra Kumari Singhdeo Government Medical College, Ambikapur-497001, Chhattisgarh. 3 Assistant Professor, Department of Conservative Dentistry and Endodontics, Dr.D.Y.Patil Dental College and Hospital, Dr.D.Y.Patil Vidyapeeth,Pimpri,Pune-18 4 Senior Lecturer, Department of Oral Pathology and Microbiology, Mansarovar Dental College Hospital and Research Centre, Bhopal, Madhya Pradesh 5 Assistant professor, Department of Orthodontics and Dentofacial Orthopaedics, Narsinhbhai Patel Dental College and hospital, Sankalchand Patel University, Visnagar, Gujarat, India 6 MBBS, MD General Medicine, MNR Medical College and Hospital, Telangana, India. DOI: 10.47750/pnr.2022.13.S06.169 Background and objectives: Controlling mechanical plaque is a crucial component of periodontal treatment. In the current research, the effectiveness of using ozone water irrigation in addition to scale and root planing to treat generalised chronic periodontitis was assessed. Materials and methods: After scaling and root planing, the twenty-four chronic periodontitis patients chosen for the research were randomly assigned to Group A or Group B, getting irrigation with ozone water or distilled water, respectively. To assess the "red complex" periodontal pathogens, subgingival plaque was taken from the chosen investigational teeth and examined using the BANA-ZymeTM Processor. At baseline, 14 days, 21 days, and 2 months, the clinical and microbiological data were noted. Results: At the baseline, Group A and Group B's mean probing pocket depth scores were 6.833 1.193 and 7.833 1.276, respectively; on Day 14, they were 6.616 1.403 and 7.083 1.378; on Day 21, they were 5.166 0.937 and 6.083 1.443; and on Month 2, they were 4.500 0.797 and 5.166 1.029. In Group A, 9 samples at the second month showed BANA negativity, 3 samples showed BANA positivity, and in Group B, 12 samples at the second month showed BANA negativity, 0 samples showed BANA positivity. In both groups, the microbiological study revealed a decrease in periodontal infections. Conclusion: An effective complement to scaling and root planing in the treatment of chronic periodontitis, subgingival ozonated water irrigation has shown significant improvement in both clinical and microbiological indices. Keywords: BANA, chronic periodontitis, Ozonated water, scaling and root planing. INTRODUCTION A polymicrobial infectious condition called chronic periodontitis results from an expansion of the microflora. The most significant pathogens of chronic periodontitis are members of the "red complex," which include Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. These various organisms are known to be associated with the clinical development of the illness. [1] Mechanical removal of dental plaque is a crucial component of managing and treating periodontal disease, and it may be done by a professional or a patient at home. To achieve total remission, it is necessary to alter the subgingival environment in order to prevent and impede the proliferation of the extremely anaerobic subgingival microflora. [2] This may be accomplished using a variety of techniques, such as the use of oxygenating and redox agents, molecular oxygen, hyperbaric oxygenation, hydrogen peroxide, and, more recently, the subgingival irrigation with ozonized water. [3] Recently, ozone has drawn interest in the realm of dentistry due to its potent antibacterial qualities that don't cause germ resistance. Therefore, the purpose of this research was to assess the effectiveness of ozone as a supplement to scale and root planing in the management of chronic periodontitis.