Study of Anticancer and Antibacterial Activities of Podophyllum Hexandrum as Natural Curatives Javaid Aftab 1 , Ejaz Aziz 2 , Asad Zulqarnain 1 , Mubeen Tabish Nasim 1 , Aamir Sajjad 1 ,Muhammad Qamrosh Zafar 1 and Riffat Batool 1 * 1 University Institute of Biochemistry and Biotechnology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Rawalpindi, Punjab 46000, Pakistan 2 Department of botany, GDC Khanpur, Haripur, Pakistan Introduction Antimicrobial obstruction (AMO) is a genuine wellbeing concern that has added to increases in horribleness, mortality, and human services expanse. Strains from different clinical and natural sources, essential cells for medicate danger screening studies and media and reagents to help cell growth are perfect for the advancement, check, and assessment of quick discovery strategies, imaginative remedial procedures, novel anti-toxins, and refreshed sterility conventions. Because of enzymatic inactivation for example aminoglycoside altering proteins, and β-lactamases. The extract with different compounds from therapeutic plants have been tried and demonstrated the viability of conventional herbs against microorganisms; thus, plants are one of the bedrocks for present day drug to accomplish new standards [1]. Accordingly, restorative plants can offer a riches for their organic exercises, for example, antimicrobial, cancer prevention agents, antimalarial, and anticancer exercises. Cancer is a deadly sickness in entire world with high pace of death cause and psychological state. As per the count of 2012, 14.1 million new instances of disease appeared in worldwide because of which 8.2 million passing’s happened [2]. Modification of base and other hereditary changes happen because of increment in level of receptive oxygen species. These species are framed from extra cell and intracellular sources. These species consolidate with deoxyribonucleic corrosive and cell proteins [3]. These redesigning causes an enormous change in the ordinary procedure of apoptosis which causes the undesirable and boundless cell division and furthermore prompts tumor development [4]. Organized researches depicted that development and progression of cancer results from intense multiplication of abnormal cells. During the process of cell division of cancer cell, many dynamic aberrations emerge in genome and accumulation of such genetic abnormalities direct the transfer of normal cells to tumor cancer cell [5]. Natural products are considered generally as the rich wellspring of phytochemicals with different bio-structures and strong bioactivities against various ailments including malignant growth and irresistible maladies [6]. In spite of the fact that the chemotherapeutic medications are the great alternative to cure from malignant growth, however they are not Crimson Publishers Wings to the Research Research Article *Corresponding author: Riffat Batool, University Institute of Biochemistry and Biotechnology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Rawalpindi, Punjab 46000, Pakistan Submission: December 18, 2019 Published: January 21, 2020 Volume 5 - Issue 5 How to cite this article: Javaid A, Ejaz Aziz Asad Z, Mubeen T N, Aamir S, Muhammad Q, . Study of Anticancer and Antibacterial Activities of Podophyllum Hexandrum as Natural Curatives. Adv Complement Alt Med. 5(5). ACAM.000621.2020. DOI: 10.31031/ACAM.2020.05.000621 Copyright@ Riffat Batool. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use and redistribution provided that the original author and source are credited. 482 Advances in Complementary & Alternative Medicine Abstract The utilization of medicinal plants to treat infectious disease is a common practice in developing countries worldwide. The present study was aimed at evaluating the crude extracts of Podophyllum hexandrum with different chemicals for anticancer and antibacterial potential. The results revealed that the methanol fractions of P. hexandrum showed significant cytotoxic potential against MCF-7 with lowest %IC50 of 41±1.79 than other extracts used in this study. Furthermore, crude extracts were also analyzed for antibacterial potential against six multi drug resistant (MDR) pathogens by disc diffusion method. The n-hexane extract showed good inhibitory effect against all the tested strains except S. typhi. Its highest activity was observed against E. coli with zone of inhibition of 22±1.25 (MIC=25g/mL). Methanol and ethanol extract also depicted inhibitory potential for most of the tested bacterial strains. In summary, P. hexandrum extracts possess antibacterial and anticancer activities, suggested to be a strong pharmaceutical agent. Keywords: Mcf-7; Podophyllum hexandrum; Antibacterial; Medicinal plants; Cytotoxic ISSN: 2637-7802