Adsorption of polyphosphoric acid to titanium surface and its effect on hBMSC attachment K. Maekawa a, * , A. Mine a , T. Fujisawa a , Y. Yoshida b , K. Suzuki b , T. Kuboki a a Department of Oral and Maxillofacial Rehabilitation, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama, 700-8558, Japan b Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan Abstract. The aim of this study was to evaluate the effect of pre-treatment of titanium (Ti) surface with polyphosphoric acid on the initial attachment of human bone marrow derived mesenchymal stem cells (hBMSCs). The results of this study revealed that adsorption of polyphosphoric acid to Ti significantly enhanced initial attachment of hBMSCs. D 2005 Elsevier B.V. All rights reserved. Keywords: Titanium; Polyphosphoric acid; Bone marrow derived mesenchymal stem cell 1. Introduction Recently, numerous attempts have been done to improve implant surface modification to attain acceleration of the osteogenic cell behaviors; however, none has been clinically accepted yet. In this situation, evidences have been provided that polyphosphate has a great variety of biological effects, including accelerating mitogenic activity of fibroblast growth factors [1]. Therefore, we evaluated the effect of polyphosphoric acid adsorption on initial cell attachment of human bone marrow derived mesenchymal stem cells. 2. Materials and methods 2.1. Polyphosphoric acid adsorption onto Ti disks Polyphosphoric acid was absorbed to Ti disk (Grade-II, 15 mm in diameter by 1 mm in thickness) surface in various concentrations (0, 0.1, 1, 10 wt.%) by im- 0531-5131/ D 2005 Elsevier B.V. All rights reserved. doi:10.1016/j.ics.2005.06.091 * Corresponding author. Tel.: +81 86 235 6620; fax: +81 86 235 6684. E-mail address: maekawa@md.okayama-u.ac.jp (K. Maekawa). International Congress Series 1284 (2005) 332 – 333 www.ics-elsevier.com