Submit Manuscript | http://medcraveonline.com Abbreviations: DETAPAC, diethylenetriaminopentaacetic acid; CDNB, 1-chloro-2,4-dinitrobenzene; EDTA, ethylenediaminetertaacetic acid; DTNB, 5,5-dithiobis(2-nitrobenzoic acid); NBT, nitrobluetetrazolium; PMS, phenazine methosulphate; TBA, 2-thiobarbituric acid; SDS, sodium dodecyl sulphate; NADH, nicotinamide adenine dinucleotide; NADPH, nicotinamide adenine dinucleotide phosphate; TNB, 5-thio-(2-nitrobenzoic acid)] Introduction The plants and various microbes synthesize various secondary metabolites having complex chemical structure. Many of these secondary metabolites have formed the basis of numerous pharmacological agents. 1,2 The screening of plants has provided an avenue for new drug discovery and many of the modern drugs have been isolated from the natural products. 3–5 The modern chemotherapy drugs have been very useful in cancer treatment however, their constant use leads to acquired drug resistance. 6,7 The complementary and alternative medicinal systems as treatment modalities is very old and they have been followed in various countries. 8 The cancer patients also use these systems for healthcare in different countries, which emphasizes the importance of these system. 9–13 This has given an impetus to screen natural products for new drug discovery. Helicia nilagirica Bedd. or Pasaltakaza in Mizo language (Family: Proteaceae) grows in lowlands to montane rain forests, up to an altitude of 1000 to 2,000 meters. It is also found along the streams, hilltops and ridges. 14 H. nilagirica is traditionally used by tribals of Mizoram, India to treat indigestion, stomach ailments, peptic ulcers, gynaecological disorders, mouth ulcers and urinary tract infections. H. nilagirica is also used in the treatment of scabies and some skin disorders. 15,16 H. nilagirica fruits are used in Sikkim to provide relief to cure cough and cold. 15,16 There has been a recent report regarding the anti-inflammatory activity of H. nilagirica. 17 The H. nilagirca aqueous extract has been reported to exert antineoplastic activity in vitro and in Dalton’s lymphoma ascites cells in tumorized mice. 15,16 However, the systematic scientific evaluation of antioxidant enzymes and lipid peroxidation of H. nilagirica has not been studied. Therefore, the present study was performed to obtain an insight into the biochemical profile of Dalton’s lymphoma ascites tumour in Swiss albino mice treated with aqueous extract of Helicia nilagirica. Materials and methods Chemicals Glutathione (reduced), diethylenetriaminopentaacetic acid (DETAPAC), 1-chloro-2,4-dinitrobenzene (CDNB), ethylenediaminetertaacetic acid (EDTA), 5,5-dithiobis(2- nitrobenzoic acid) (DTNB), nitrobluetetrazolium (NBT), phenazine methosulphate (PMS), sodium azide, 2-thiobarbituric acid (TBA), sodium dodecyl sulphate (SDS), nicotinamide adenine dinucleotide (NADH), nicotinamide adenine dinucleotide phosphate (NADPH), MOJ Anat & Physiol. 2019;6(4):135‒143. 135 ©2019 Jagetia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and build upon your work non-commercially. Alteration in the activities of antioxidant enzymes and lipid peroxidation in mice transplanted with Dalton’s lymphoma ascites tumor by Helicia nilagirica Bedd Volume 6 Issue 4 - 2019 Ganesh Chandra Jagetia, Jennifer Zoremsiami Department of Zoology, Mizoram University, India Correspondence: Ganesh Chandra Jagetia, 10, Maharana Pratap Colony, Sector-13, Hiran Magri, Udaipur-313002, India, Email Received: May 24, 2019 | Published: August 22, 2019 Abstract The present study was performed to obtain an insight into the biochemical profile of Dalton’s lymphoma ascites tumour bearing Swiss albino mice treated with aqueous extract of Helicia nilagirica. The mice bearing Dalton’s lymphoma ascites tumor was injected with 175mg/kg body weight of aqueous extract of H. nilagirica for nine consecutive days. Thereafter, the tumour cells were aspirated at 2, 4, 6, 8, 12 and 24h post drug treatment for the estimation of glutathione, glutathione-s-transferase, catalase, superoxide dismutase, and lipid peroxidation. The administration of tumorized mice with H. nilagirica aqueous extract caused a significant depletion in the glutathione contents and activities of glutathione-s- transferase, catalase, superoxide dismutase in a time dependent manner up to 24 h post assay time, when compared to sterile physiological saline treated group. In contrast, treatment of tumorized mice with H. nilagirica aqueous extract resulted in a time dependent rise in the lipid peroxidation when compared to sterile physiological saline treated group. Our study demonstrates that the cytotoxic effect of H. nilagirica aqueous extract on Dalton’s lymphoma ascites tumour cells may be due to increased lipid peroxidation and reduction in the glutathione contents, activities of glutathione-s-transferase, catalase, and superoxide dismutase. Keywords: mice, Helicia nilagirica, dalton’s lymphoma, glutathione, antioxidant enzymes, lipid peroxidation MOJ Anatomy & Physiology Research Article Open Access