Pure Appl. Biol., 12(1):623-636, March, 2023 http://dx.doi.org/10.19045/bspab.2023.120064 Published by Bolan Society for Pure and Applied Biology 623 Research Article Allelopathic assessment ofTrianthema portulacastrum L.against germination and early growth of weeds in wheat Muhammad Shahid Hassan 1* , Nargis Naz 1 , Muhammad Akram 1 , Mumtaz Hussain 2 and Hassan Raza Javeed 1 1. Department of Botany, The Islamia University of Bahawalpur, Punjab, Pakistan 2. Department of Anatomy and Histology, Faculty of Veterinary Sciences, The Islamia University of Bahawalpur, Punjab, Pakistan *Corresponding author’s email:mshassan2015@gmail.com Citation Muhammad Shahid Hassan, Nargis Naz, Muhammad Akram, Mumtaz Hussain and Hassan Raza Javeed. Allelopathic assessment of Trianthema portulacastrum L. against germination and early growth of weeds in wheat. Pure and Applied Biology. Vol. 12, Issue 1, pp623-636. http://dx.doi.org/10.19045/bspab.2023.120064 Received: 01/10/2022 Revised: 16/12/2022 Accepted: 20/12/2022 Online First: 30/12/2022 Abstract Weeds are one of the key threats to the natural and agricultural fields. Weeds pose severe losses in agricultural yield by procuring nutrients and releasing secondary metabolites. Therefore, weed management is crucial for securing quantity and quality of yield. Various strategies that are most commonly employed to weed control are mechanical, physical, chemical, and cultural. However, the best method to eradicate weeds is a biological approach. The herbicidal potential of plants that can produce allelochemicals is considered a major source of biological control.In crops, weed control through allelopathy is a beneficial and environment-friendly alternativeto traditional herbicides.The object of this study is to explore the allelopathic impact of Trianthema portulacastrum against the growth of weeds (Asphodelus tenuifolius and Convolvulusarvensis and wheat(Triticum aestivum). The experimentwas performed in the petri-plates to explore the effect of various concentrations (30%, 60%, and 100%) of root and shoot of T. portulacastrum and herbicide (Metafin Super 28.6% WDG) along with control (distilled water)on the seed germination and growth of the weeds and wheat. To meet up the objectives, various parameters (such as seed germination, root length, shoot length, and seedling dry weight)of tested species were estimated. Our results revealed that water extracts of T. portulacastrum significantly reducedthe growth parameterspossibility due to interference with various growth metabolic processes. We conclude thatwater extract of root and shootof T. portulacastrumcould be safer and more beneficial to minimize the weeds competition in the wheat field. Keywords: Allelopathic extract; Dry weight; Petri-plates; Root length; Trianthema portulacastrum; Weeds; Wheat Introduction Several factors cause low wheat yield in Pakistan, among these major intimidations to crop productivity, is an imbalance in crop nutrition, weeds, diseases, pests, and abiotic stresses, and exploration of these limiting factors will surely result in utmost crop yield [1]. In natural and agrarian fields, weeds are one of the key intimidations. Almost 30,000 weed species have been recognized in the