Original Article This work is licensed under the Creative Commons Attribution 4.0 License. Published by Pacific Group of e-Journals (PaGe) Aerobic Bacteriological Profile and its Antimicrobial Sensitivity Pattern From Blood Culture Specimens in A Tertiary Care Hospital. DOI: 10.21276/apalm.2017.988 Introduction Blood stream infections (BSIs) are important cause of mortality and morbidity and are among most common health-associated infections [1] .Bacteremia signifies the presence of bacteria in the blood stream [1] .Bacteremia may be transient, continuous or intermittent. Micro-organisms present in the circulating blood are a threat to every organ in the body [1] . It can have serious consequences like shock, multiple organ failure, disseminated intravascular coagulation, etc. thus, the blood stream infection constitutes one the most serious situations with increased cost of care, morbidity, mortality and , as a result,timely detection and identification of blood stream pathogen is important. Blood culture plays an integral role in the evaluation of sepsis [1,2] . Increasing antimicrobial resistance is worldwide concern. The prevalence of resistance in both out-patients and hospitalised patients with septicaemia is increasing, and its varies in accordance with geographical and regional location. In almost all cases, antimicrobial therapy is initiated empirically before the results of blood culture are available [3] . Selecting appropriate antimicrobial for treating BSI is multifaceted, including possible cause and source of infection, in vitro activity of drug according to microbiological susceptibility testing results, pharmacokinetics and adverse effects of the drug [2] . However, before considering these aspects, the choice of antibiotic mainly relies on knowledge of the pathogen likely involved.Monitoring and analysing the antimicrobial suscepitibility pattern of most frequently isolated micro- organisms according to local epidemiology which helps clinicians to choose empirical therapies and develop rational prescription policy for antibiotics [4,5] . Therefore, the present study was undertaken to describe aerobic bacteriological profile and its antibiotic sensitivity pattern from blood culture specimen in a tertiary care setting to guide clinicians to initiate empiric antibiotic therapy and to formulate antibiotic policy. Materials and Methods Study Design: The present study is prospective type of Santosh A. Kotgire*, Sunil Hatkar S Department of Microbiology, Indian Institute of Medical Sciences, Jalna, Maharashtra, India ABSTRACT Background :Blood stream infections (BSIs) are important cause of mortality and morbidity and are among most common health-associated infections.Blood stream infection constitutes one the most serious situations with increased cost of care, morbidity, mortality and thus, timely detection and identification of blood stream pathogen is important.Sothe present study was undertaken to describe aerobic bacteriological profile and its antibiotic sensitivity pattern from blood culture specimen in a tertiary care setting. Method: The study was carried out in the Department of Microbiology, Indian Institute of Medical Sciences, Jalna, Maharashtra for a period of 8 months (July 2015 to February 2016). A total 1920 samples were evaluated from clinically suspected cases of bacteremia. Blood was collected depending upon age group with aseptic precaution and inoculated on brain heart infusion broth (BHIB).Subculture were made on blood agar and Mac-conkey agar plates. Organisms were identified and antibiotic sensitivity test of isolates were performed. Result : During 8 month study period out of 1920 blood culture, 369(19.21%) yielded growth of different organisms. Out of this 369 organisms gram positive bacteria 199 (53.9%) were isolated more often than gram negative bacteria 170 (46.1%). Staphylococcus aureus 49.05 % was leading pathogens isolated followed by enterobactericae group 21.00% (Escherichia coli and Klebsiella spp) and Salmonella typhi & S. Paratyphi A 13.82%. Conclusion: This study provides information on antibiotic sensitivity pattern of blood isolates which may be useful to guide clinicians to initiate empiric therapy and will help in formulation of antibiotic therapy strategy in this part of country. Keywords: Blood Culture, Antimicrobial Sensitivity Pattern, Bacteremia. study and was carried out at Department of Microbiology,