EMAS2017 / Maturitas 100 (2017) 93–202 177 PCO: 15% (most prevalent endocrine disorder) Local therapies have low and medium efﬁcacy. The combination of estrogen with an antiandrogenic progesto- gen is the most effective treatment targeting symptoms and endocrine dysregulation. The individual cardiovascular risk has to be assessed and balanced with the beneﬁt. http://dx.doi.org/10.1016/j.maturitas.2017.03.197 P098 Analysis of Codon 72 polymorphism in the p53 tumor suppressor gene in women with idiopathic recurrent pregnancy loss Sang Ho Yoon Dongguk University Ilsan Medical Center, Obstetrics and Gynecology, Goyang, Republic of Korea Objective: The balance of apoptosis and proliferation is an important part in the embryonic development during pregnancy. It has been reported that the p53 gene plays a signiﬁcant role in angiogenesis and placental development, namely in reproduction and is suggested as a potential mediator of pregnancy. This study was performed to investigate whether the genetic polymorphism of the p53 gene is associated with idiopathic recurrent pregnancy loss (RPL). Study design: We conducted a case-control study. Study sub- jects consisted of 294 patients with idiopathic RPL and 300 postmenopausal controls. The genotyping for the p53 codon 72 polymorphism was performed using a Taqman assay. Continuous variables were compared using Student’s t test and the  2 test was used to evaluate differences in the genotype distributions between the RPL and the controls. Results: The median number (range) of spontaneous miscar- riages was 3 (2–13) in RPL patients and 0 (0-0) in controls. There were no signiﬁcant differences in the genotype distributions or allele frequencies of the p53 codon 72 polymorphism between the RPL and control group (Arg/Pro rates; 65.3/34.7% in the RPL vs. 64.8/35.2% in the control group, p = 0.864). There was also no sig- niﬁcant association between the p53 codon 72 polymorphism and RPL risk in both recessive (Pro/Pro vs. Arg-carriers, p = 0.314) and dominant model (Pro-carriers vs. Arg/Arg, p = 0.383). For further analysis, if RPL patients were divided according to the numbers of pregnancy losses (≥2 and ≥3), neither group was signiﬁcantly different compared with controls. Conclusions: The codon 72 polymorphism in the p53 gene did not show any correlation with idiopathic RPL in Korean women, implying that it may not be susceptible allelic variants or be insuf- ﬁcient to cause RPL. Keywords: Codon 72 polymorphism; p53 tumor suppressor gene; Recurrent pregnancy loss http://dx.doi.org/10.1016/j.maturitas.2017.03.198 P099 Application of robotic single-site surgery for early stage cervical cancer: A pilot study Sang Hoon Kwon ∗ , Chi Heum Cho Keimyung University, Daegu, Republic of Korea Aims: Recent reports propose that robotic single-site (RSS) surgery is feasible in treating a benign condition of gynecologic pro- cess. The aim of this study is to evaluate the feasibility and safety of RSS surgery for the surgical treatment of early stage cervical cancer (carcinoma in situ of cervix & cervical cancer Ia1). Method: Patients with preoperative diagnosis of CIS and cer- vical cancer Ia1 by loop electrosurgical excision procedure were selected. 12 patients (10 patients: CIS, 2 patients: cervical cancer Ia1) who underwent RSS surgery from March 2014 to August 2015, at Dongsan medical center, were included in this study. All surgi- cal procedures were performed by robotic single-site instruments (da Vinci Si ® surgical System, Sunnyvale, CA) via a single umbili- cal incision. All patients underwent type 1 hysterectomy with or without salpingo-oophorectomy according to the grossly ovarian pathology. Results: The Median patient age and body mass index were 42.5 years (range, 33–61 years) and 22.2 kg/m 2 (range, 18.6–26.4 kg/m 2 ). The median console time and total opera- tive time was 52.5 min (range, 36–185 min) and 125 min (range, 90–280 min), respectively. There was no case of conversion to laparoscopy or laparotomy. There was only one wound disrup- tion and dehiscence of umbilical skin. Patients were repaired at post-operative 1month. Conclusion: RSS surgery is feasible and safe in selected patients with early stage cervical cancer. Operative times were reasonable and surgical procedure was well tolerated by patients. Large-scaled studies comparing laparoscopic single site surgery in patients should be performed to conﬁrm the safety and beneﬁts of RSS surgery. http://dx.doi.org/10.1016/j.maturitas.2017.03.199 P100 Asymptomatic high grade endometrial cancer Laura Baquedano 1,∗ , Pluvio Jesús Coronado 2 , Maria Angeles Martinez-Maestre 3 , Yasmina José 1 , Miguel Angel Ruiz-Conde 1 , Diego Judez 4 1 Hospital Universitario Miguel Servet, Zaragoza, Spain 2 Hospital Clinico San Carlos, Madrid, Spain 3 Hospital Virgen del Rocío, Sevilla, Spain 4 Hospital Alca˜niz, Teruel, Spain Most women with endometrial cancer (EC) have abnormal uter- ine bleeding. However, there is a small percentage of patients who are asymptomatic at the time of diagnosis. The objective of the study is to analyze the epidemiological characteristics and risk fac- tors of asymptomatic high grade EC. Material and methods: Retrospective multicentric cohort study in 3 Spanish hospitals: Hospital Universitario Miguel Servet in Zaragoza, Hospital Clínico San Carlos in Madrid and Hospital Virgen del Rocío in Sevilla. We studied the epidemiological characteris- tics and the presence of risk factors associated with high grade EC depending on the presence of symptoms at the time of diagnosis. We included all subtypes of high grade EC: G3 endometrioid (G3EC), serous carcinoma (SC), clear cell carcinoma (CCC) and malignant mixed mesodermal tumors (MMMT). A total of 373 cases of high- grade EC were included (135 G3EC, 96 SC, 64 CCC and 78 MMMT). Results: 90% of all patients (n = 335), had uterine bleeding at the time of EC diagnosis. All patients with asymptomatic EC (n = 37) were postmenopausal; of them, 24.3% (n = 9) were nulligest, 45.9% (n = 17) hypertensive, 45.9% (n = 17) were obese and 2.7% (n = 1) were under hormonal treatment. There were no signiﬁcant differences between patients with asymptomatic high-grade carci- noma and those who present clinic in these factors: hypertension (p = 0.433), diabetes (p = 0.577), obesity (p = 0.825), use of hormone