Sapporo Medical Journal Volum 56, Issue 05, May 2022 1 Spectrophotometric Determination of Nitazoxinide Antiviral Drugs by Reaction of Diazonium salt Riyadh R. Al-Araji 1 , Muthana S.Mashkour 1 , Rahman S. Zabibah 2 Department of Chemistry, Faculty of Science, University of Kufa, Iraq 1 Department of Medical Laboratory Technology, College of Medical Technology, Islamic University, Najaf, Iraq. 2 Abstract— Spectrophotometric techniques can be used to accurately and rapidly determine the concentration of Nitazoxanide in body fluids (serum and urine) through react with diazonium salt ,which result from hydrolyze amino group of 2-Aminobenzothiazole by hydrochloric acid , to formation a precipitate a yellow-orange azo dye. Showed obeyed beers law between 50- 450 μg.mL -1 , Molar absorptivity of 0.4916 x10 3 L mol -1 /cm -1 was measured at 478 nm, . for Sandal's sensitivity 0.0132 has a limit of detection (LOD) of 2.96 μg.mL -1 and a limit of quantification (LOQ) of 9.875 μg.mL -1 . This technique has been successfully used to quantify various concentrations of Nitazoxanide in body fluids. Keywords: Nitazoxanide Antiviral (NTZ), Diazotization, Spectrophotometry, 2- aminobenzothyazol 1. Introduction Drugs with new modes of action that may be employed alone or in conjunction with neuraminidase inhibitors are needed in order to offer more effective medical treatment for seasonal and pandemic influenza. As a result, new influenza strains are always on the rise, and the present therapeutic options are unable to combat them effectively. A significant contribution to this field might be made by the redistribution of Nitazoxanide as an influenza therapy. It has been discovered via research into The mechanism of action of Nitazoxanide against influenza viruses is that the medication interferes with the maturation of the viral hemagglutinin during the post-translational stage of the virus's replication cycle [1].as a method of providing stronger protection against a broader range of viruses while avoiding the dangers of resistance and reducing the costs associated with developing treatments that are tailored to each specific virus type [2,3]. Use to treat and prevent a broad range of protozoa, parasites, and gram-negative organisms, Nitazoxanide is the most often used antiprotozoal and antihelmintic agent. Intestinal protozoal diseases as well as helminthiasis are both treated . All patients with impaired immune systems, including those with HIV or AIDS, Giardia lamblia-caused diarrhea .Antiprotozoal effectiveness may be based on NTZ's capacity to disrupt the PFOR enzyme-dependent electron transfer mechanism, which is essential to anaerobic energy metabolism, which NTZ inhibits. Nitazoxanide (NTZ) is a novel antiparasitic and antiprotozoal compound with a wide range of action against a variety of parasites and protozoa. It is a nitrothiazole derivative with the chemical name 2-acetyloxyl-N-(5-nitro-2-thiazolyl) benzamide as its chemical name. It is a nitrothiazole derivative (Fig. 1) [4].